A calcium channel reversibly blocked by ω-conotoxin GVIA lacking the class D α1 subunit

Paul G. Mermelstein; D. James Surmeier

doi:10.1097/00001756-199701200-00022

A calcium channel reversibly blocked by ω-conotoxin GVIA lacking the class D α₁ subunit

Paul G. Mermelstein, D. James Surmeier^*

^*Corresponding author for this work

Neuroscience

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

In acutely dissociated rat medium-spiny neostriatal neurons, a substantial fraction (~40%) of the calcium current block by ω-conotoxin-GVIA (CgTx) reverses rapidly with washing. The reversibly blocked current differed in voltage dependence from the current irreversibly blocked by CgTx. To test the hypothesis that reversibly blocked current was attributable to calcium channels with a class D α₁ subunit, acutel isolated neurons were subjected to combined patch clamp/reverse transcription-polymerase chain reaction analysis. These studies revealed no correlation between the reversibility of the CgTx block and the expression of class D α₁ subunit mRNA.

Original language	English (US)
Pages (from-to)	485-489
Number of pages	5
Journal	Neuroreport
Volume	8
Issue number	2
DOIs	https://doi.org/10.1097/00001756-199701200-00022
State	Published - 1997

Keywords

Bay K 8644
N-type calcium channels
Patchclamp
RT-PCR
Striatum

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1097/00001756-199701200-00022

Cite this

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title = "A calcium channel reversibly blocked by ω-conotoxin GVIA lacking the class D α1 subunit",

abstract = "In acutely dissociated rat medium-spiny neostriatal neurons, a substantial fraction (~40%) of the calcium current block by ω-conotoxin-GVIA (CgTx) reverses rapidly with washing. The reversibly blocked current differed in voltage dependence from the current irreversibly blocked by CgTx. To test the hypothesis that reversibly blocked current was attributable to calcium channels with a class D α1 subunit, acutel isolated neurons were subjected to combined patch clamp/reverse transcription-polymerase chain reaction analysis. These studies revealed no correlation between the reversibility of the CgTx block and the expression of class D α1 subunit mRNA.",

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T1 - A calcium channel reversibly blocked by ω-conotoxin GVIA lacking the class D α1 subunit

AU - Mermelstein, Paul G.

AU - Surmeier, D. James

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AB - In acutely dissociated rat medium-spiny neostriatal neurons, a substantial fraction (~40%) of the calcium current block by ω-conotoxin-GVIA (CgTx) reverses rapidly with washing. The reversibly blocked current differed in voltage dependence from the current irreversibly blocked by CgTx. To test the hypothesis that reversibly blocked current was attributable to calcium channels with a class D α1 subunit, acutel isolated neurons were subjected to combined patch clamp/reverse transcription-polymerase chain reaction analysis. These studies revealed no correlation between the reversibility of the CgTx block and the expression of class D α1 subunit mRNA.

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