A calcium-modulated plasmonic switch

W. Paige Hall, Jeffrey N. Anker, Yao Lin, Justin Modica, Milan Mrksich, Richard P. Van Duyne

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

A plasmonic switch based on the calcium-induced conformational changes of calmodulin is shown to exhibit reversible wavelength modulations in response to changing calcium concentration. The extinction maximum (λmax) of a localized surface plasmon resonance (LSPR) sensor functionalized with a novel calmodulin construct, cutinase-calmodulin-cutinase (CutCaMCut), reversibly shifts by 2-3 nm. A high-resolution (HR) LSPR spectrometer with a wavelength resolution (3σ) of 1.5 × 10-2 nm was developed to detect these wavelength modulations in real-time, providing information about the dynamics and structure of the protein. The rate of conversion from open (Ca2+-bound) to closed (Ca2+-free) calmodulin is shown to be 4-fold faster than the reverse process, with a closing rate of 0.127 s-1 and opening rate of 0.034 s-1. As far as we are aware, this plasmonic switch marks the first use of LSPR spectroscopy to detect reversible conformational changes in an unlabeled protein.

Original languageEnglish (US)
Pages (from-to)5836-5837
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number18
DOIs
StatePublished - May 7 2008

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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