TY - JOUR
T1 - A capacitive biosensor for detection of staphylococcal enterotoxin B
AU - Labib, Mahmoud
AU - Hedström, Martin
AU - Amin, Magdy
AU - Mattiasson, Bo
N1 - Funding Information:
Acknowledgment Ministry of Higher Education, Egypt is gratefully acknowledged for financial support to Mahmoud Labib during his study at the Department of Biotechnology, Lund University.
PY - 2009/3
Y1 - 2009/3
N2 - A sensitive method for the detection of staphylococcal enterotoxin B (SEB) using a flow-injection capacitive biosensor is presented. SEB was purified from a crude culture filtrate of Staphylococcus aureus through three chromatographic steps. The first two steps were based on ion-exchange chromatography, and the last step was carried out on a gel filtration column. The SEB recovery values after the purification stages were 88%, 74%, and 12%, respectively. A horseradish peroxidase labeled antistaphylococcal enterotoxin B was prepared by the periodate method and was further employed in a sandwich-enzyme-linked immunosorbent assay (ELISA) for the determination of SEB concentrations in different samples obtained during the processing of the crude filtrate. The capacitive biosensor could detect SEB concentrations as low as 0.3 pg ml -1 with a linearity ranging from 2.8 pg ml-1 to 2.8 ng ml-1 under optimized conditions. The response time was about 10 min. A good agreement was achieved between the developed capacitive biosensor system and ELISA as a reference method for detection of SEB levels in different purification samples. The newly developed sensor has the benefits of simplicity, high sensitivity, and multiple use capability.
AB - A sensitive method for the detection of staphylococcal enterotoxin B (SEB) using a flow-injection capacitive biosensor is presented. SEB was purified from a crude culture filtrate of Staphylococcus aureus through three chromatographic steps. The first two steps were based on ion-exchange chromatography, and the last step was carried out on a gel filtration column. The SEB recovery values after the purification stages were 88%, 74%, and 12%, respectively. A horseradish peroxidase labeled antistaphylococcal enterotoxin B was prepared by the periodate method and was further employed in a sandwich-enzyme-linked immunosorbent assay (ELISA) for the determination of SEB concentrations in different samples obtained during the processing of the crude filtrate. The capacitive biosensor could detect SEB concentrations as low as 0.3 pg ml -1 with a linearity ranging from 2.8 pg ml-1 to 2.8 ng ml-1 under optimized conditions. The response time was about 10 min. A good agreement was achieved between the developed capacitive biosensor system and ELISA as a reference method for detection of SEB levels in different purification samples. The newly developed sensor has the benefits of simplicity, high sensitivity, and multiple use capability.
KW - Capacitive biosensor
KW - ELISA
KW - Horseradish peroxidase
KW - Self-assembled monolayers
KW - Staphylococcal enterotoxin B
UR - http://www.scopus.com/inward/record.url?scp=60749110917&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60749110917&partnerID=8YFLogxK
U2 - 10.1007/s00216-008-2559-x
DO - 10.1007/s00216-008-2559-x
M3 - Article
C2 - 19096830
AN - SCOPUS:60749110917
SN - 0016-1152
VL - 393
SP - 1539
EP - 1544
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
IS - 5
ER -