A cell-based approach to the human proteome project

Neil L. Kelleher*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The general scope of a project to determine the protein molecules that comprise the cells within the human body is framed. By focusing on protein primary structure as expressed in specific cell types, this concept for a cell-based version of the Human Proteome Project (CB-HPP) is crafted in a manner analogous to the Human Genome Project while recognizing that cells provide a primary context in which to define a proteome. Several activities flow from this articulation of the HPP, which enables the definition of clear milestones and deliverables. The CB-HPP highlights major gaps in our knowledge regarding cell heterogeneity and protein isoforms, and calls for development of technology that is capable of defining all human cell types and their proteomes. The main activities will involve mapping and sorting cell types combined with the application of beyond the state-of-the art in protein mass spectrometry.

Original languageEnglish (US)
Pages (from-to)1617-1624
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume23
Issue number10
DOIs
StatePublished - Oct 2012

Keywords

  • Cell type
  • Human genome project
  • Human proteome project
  • Mass spectrometry
  • Protein isoforms
  • Top down proteomics

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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