A cell separation checkpoint that enforces the proper order of late cytokinetic events

Jennifer L. Brace, Matthew D. Doerfler, Eric L Weiss

Research output: Contribution to journalArticle

Abstract

Eukaryotic cell division requires dependency relationships in which late processes commence only after early ones are appropriately completed. We have discovered a system that blocks late events of cytokinesis until early ones are successfully accomplished. In budding yeast, cytokinetic actomyosin ring contraction and membrane ingression are coupled with deposition of an extracellular septum that is selectively degraded in its primary septum immediately after its completion by secreted enzymes. We find this secretion event is linked to septum completion and forestalled when the process is slowed. Delay of septum degradation requires Fir1, an intrinsically disordered protein localized to the cytokinesis site that is degraded upon septum completion but stabilized when septation is aberrant. Fir1 protects cytokinesis in part by inhibiting a separation-specific exocytosis function of the NDR/LATS kinase Cbk1, a key component of "hippo" signaling that induces mother-daughter separation. We term this system enforcement of cytokinesis order, a checkpoint ensuring proper temporal sequence of mechanistically incompatible processes of cytokinesis.

LanguageEnglish (US)
Pages150-170
Number of pages21
JournalThe Journal of cell biology
Volume218
Issue number1
DOIs
StatePublished - Jan 7 2019

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Cytokinesis
Cell Separation
Long-Acting Thyroid Stimulator
Intrinsically Disordered Proteins
Actomyosin
Saccharomycetales
Exocytosis
Eukaryotic Cells
Cell Division
Phosphotransferases
Membranes
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Brace, Jennifer L. ; Doerfler, Matthew D. ; Weiss, Eric L. / A cell separation checkpoint that enforces the proper order of late cytokinetic events. In: The Journal of cell biology. 2019 ; Vol. 218, No. 1. pp. 150-170.
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A cell separation checkpoint that enforces the proper order of late cytokinetic events. / Brace, Jennifer L.; Doerfler, Matthew D.; Weiss, Eric L.

In: The Journal of cell biology, Vol. 218, No. 1, 07.01.2019, p. 150-170.

Research output: Contribution to journalArticle

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