A characterisation of dark-field imaging of colloidal gold labels in a scanning transmission X-ray microscope

Henry N. Chapman; Chris Jacobsen; Shawn Williams

doi:10.1016/0304-3991(96)00003-4

A characterisation of dark-field imaging of colloidal gold labels in a scanning transmission X-ray microscope

Henry N. Chapman^*, Chris Jacobsen, Shawn Williams

^*Corresponding author for this work

Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Using scanning transmission X-ray microscope, high-angle dark-field images of cells with colloidal gold labels, with and without silver enhancement, are characterized. The high density of the colloidal gold particles, or the silver particles seeded by the gold, leads to a large scattering signal, and the fact that the particle diameters are comparable to the width of the microscope point spread function results in good localization of the label with high contrast. The dark-field images can have greater signal to noise ratio that bright-field images acquired with the same incident X-ray dose.

Original language	English (US)
Pages (from-to)	191-213
Number of pages	23
Journal	Ultramicroscopy
Volume	62
Issue number	3
DOIs	https://doi.org/10.1016/0304-3991(96)00003-4
State	Published - Jan 1 1996

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Instrumentation

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abstract = "Using scanning transmission X-ray microscope, high-angle dark-field images of cells with colloidal gold labels, with and without silver enhancement, are characterized. The high density of the colloidal gold particles, or the silver particles seeded by the gold, leads to a large scattering signal, and the fact that the particle diameters are comparable to the width of the microscope point spread function results in good localization of the label with high contrast. The dark-field images can have greater signal to noise ratio that bright-field images acquired with the same incident X-ray dose.",

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AU - Williams, Shawn

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N2 - Using scanning transmission X-ray microscope, high-angle dark-field images of cells with colloidal gold labels, with and without silver enhancement, are characterized. The high density of the colloidal gold particles, or the silver particles seeded by the gold, leads to a large scattering signal, and the fact that the particle diameters are comparable to the width of the microscope point spread function results in good localization of the label with high contrast. The dark-field images can have greater signal to noise ratio that bright-field images acquired with the same incident X-ray dose.

AB - Using scanning transmission X-ray microscope, high-angle dark-field images of cells with colloidal gold labels, with and without silver enhancement, are characterized. The high density of the colloidal gold particles, or the silver particles seeded by the gold, leads to a large scattering signal, and the fact that the particle diameters are comparable to the width of the microscope point spread function results in good localization of the label with high contrast. The dark-field images can have greater signal to noise ratio that bright-field images acquired with the same incident X-ray dose.

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A characterisation of dark-field imaging of colloidal gold labels in a scanning transmission X-ray microscope

Abstract

ASJC Scopus subject areas

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