A chemical approach to mapping nucleosomes at base pair resolution in yeast

Kristin R. Brogaard, Liqun Xi, Ji Ping Wang*, Jonathan Widom

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

21 Scopus citations

Abstract

Most eukaryotic DNA exists in DNA-protein complexes known as nucleosomes. The exact locations of nucleosomes along the genome play a critical role in chromosome functions and gene regulation. However, the current methods for nucleosome mapping do not provide the necessary accuracy to identify the precise nucleosome locations. Here we describe a new experimental approach that directly maps nucleosome center locations in vivo genome-wide at single base pair resolution.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc
Pages315-334
Number of pages20
DOIs
StatePublished - 2012

Publication series

NameMethods in Enzymology
Volume513
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cleavage pattern
  • Cysteine
  • Hydroxyl radical
  • Nucleosome
  • Nucleosome center positioning score
  • S. cerevisiae
  • Template model

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Fingerprint

Dive into the research topics of 'A chemical approach to mapping nucleosomes at base pair resolution in yeast'. Together they form a unique fingerprint.

Cite this