A chemical approach to mapping nucleosomes at base pair resolution in yeast

Kristin R. Brogaard, Liqun Xi, Ji Ping Wang*, Jonathan Widom

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Scopus citations


Most eukaryotic DNA exists in DNA-protein complexes known as nucleosomes. The exact locations of nucleosomes along the genome play a critical role in chromosome functions and gene regulation. However, the current methods for nucleosome mapping do not provide the necessary accuracy to identify the precise nucleosome locations. Here we describe a new experimental approach that directly maps nucleosome center locations in vivo genome-wide at single base pair resolution.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc
Number of pages20
StatePublished - 2012

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988



  • Cleavage pattern
  • Cysteine
  • Hydroxyl radical
  • Nucleosome
  • Nucleosome center positioning score
  • S. cerevisiae
  • Template model

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Brogaard, K. R., Xi, L., Wang, J. P., & Widom, J. (2012). A chemical approach to mapping nucleosomes at base pair resolution in yeast. In Methods in Enzymology (pp. 315-334). (Methods in Enzymology; Vol. 513). Academic Press Inc. https://doi.org/10.1016/B978-0-12-391938-0.00014-8