A cloned human CCAAT-box-binding factor stimulates transcription from the human hsp70 promoter

L. S.Y. Lum, L. A. Sultzman, R. J. Kaufman, D. I.H. Linzer, B. J. Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA clone encoding a 114-kDa polypeptide that binds to the CCAAT element of the hsp70 promoter. Expression of this CCAAT-binding factor (CBF) cDNA activated transcription from cotransfected hsp70 promoter-reporter gene constructs in a CCAAT-dependent manner. CCAAT-binding factor shows no homology to the previously identified human CCAAT transcription factor or rat CCAAT/enhancer-binding protein.

Original languageEnglish (US)
Pages (from-to)6709-6717
Number of pages9
JournalMolecular and cellular biology
Volume10
Issue number12
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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