Abstract
The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA clone encoding a 114-kDa polypeptide that binds to the CCAAT element of the hsp70 promoter. Expression of this CCAAT-binding factor (CBF) cDNA activated transcription from cotransfected hsp70 promoter-reporter gene constructs in a CCAAT-dependent manner. CCAAT-binding factor shows no homology to the previously identified human CCAAT transcription factor or rat CCAAT/enhancer-binding protein.
Original language | English (US) |
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Pages (from-to) | 6709-6717 |
Number of pages | 9 |
Journal | Molecular and cellular biology |
Volume | 10 |
Issue number | 12 |
State | Published - 1990 |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology