A combined method to quantify the retinal metabolic rate of oxygen using photoacoustic ophthalmoscopy and optical coherence tomography

Wei Song; Qing Wei; Wenzhong Liu; Tan Liu; Ji Yi; Nader Sheibani; Amani A. Fawzi; Robert A. Linsenmeier; Shuliang Jiao; Hao F. Zhang

doi:10.1038/srep06525

A combined method to quantify the retinal metabolic rate of oxygen using photoacoustic ophthalmoscopy and optical coherence tomography

Wei Song, Qing Wei, Wenzhong Liu, Tan Liu, Ji Yi, Nader Sheibani, Amani A. Fawzi, Robert A. Linsenmeier, Shuliang Jiao, Hao F. Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

Quantitatively determining physiological parameters at a microscopic level in the retina furthers the understanding of the molecular pathways of blinding diseases, such as diabetic retinopathy and glaucoma. An essential parameter, which has yet to be quantified noninvasively, is the retinal oxygen metabolic rate (rMRO₂). Quantifying rMRO₂ is challenging because two parameters, the blood flow rate and hemoglobin oxygen saturation (sO₂), must be measured together. We combined photoacoustic ophthalmoscopy (PAOM) with spectral domain-optical coherence tomography (SD-OCT) to tackle this challenge, in which PAOM measured the sO₂ and SD-OCT mapped the blood flow rate. We tested the integrated system on normal wild-type rats, in which the measured rMRO₂ was 297.86 ± 70.23 nl/minute. This quantitative method may shed new light on both fundamental research and clinical care in ophthalmology in the future.

Original language	English (US)
Article number	6525
Journal	Scientific reports
Volume	4
DOIs	https://doi.org/10.1038/srep06525
State	Published - 2014

Funding

This work is supported in part by the NIH grants 1R01EY019951 and 1R24EY022883. It is also supported in part by the NSF grants CBET-1055379, DBI-1353952, and CBET-1066776. W. Song was partially supported by the China Scholarship Council. W. Liu is supported by the Howard Hughes Medical Institute International Student Research Fellowship.

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abstract = "Quantitatively determining physiological parameters at a microscopic level in the retina furthers the understanding of the molecular pathways of blinding diseases, such as diabetic retinopathy and glaucoma. An essential parameter, which has yet to be quantified noninvasively, is the retinal oxygen metabolic rate (rMRO2). Quantifying rMRO2 is challenging because two parameters, the blood flow rate and hemoglobin oxygen saturation (sO2), must be measured together. We combined photoacoustic ophthalmoscopy (PAOM) with spectral domain-optical coherence tomography (SD-OCT) to tackle this challenge, in which PAOM measured the sO2 and SD-OCT mapped the blood flow rate. We tested the integrated system on normal wild-type rats, in which the measured rMRO2 was 297.86 ± 70.23 nl/minute. This quantitative method may shed new light on both fundamental research and clinical care in ophthalmology in the future.",

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AU - Wei, Qing

AU - Liu, Wenzhong

AU - Liu, Tan

AU - Yi, Ji

AU - Sheibani, Nader

AU - Fawzi, Amani A.

AU - Linsenmeier, Robert A.

AU - Jiao, Shuliang

AU - Zhang, Hao F.

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A combined method to quantify the retinal metabolic rate of oxygen using photoacoustic ophthalmoscopy and optical coherence tomography

Abstract

Funding

ASJC Scopus subject areas

UN SDGs

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