A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105

Guillermo Oliver, Fernando Valle, Francisco Rosetti, Marisa Gomez-Pedrozo, Patricia Santamaría, Guillermo Gosset, Francisco Bolivar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Penicillin acylase (PA) is an industrial enzyme that is used to convert penicillin G into a precursor for semisynthetic penicillins. We have cloned a segment of DNA that codes for the two subunits required for PA activity. We also report the nucleotide sequence of a DNA fragment that codes for (i) the small subunit, (ii) the N-terminal region of the large subunit and (iii) a putative connecting peptide. These results confirm the existence of a common precursor for both peptides.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalGene
Volume40
Issue number1
DOIs
StatePublished - 1985

Keywords

  • DNA sequence
  • Recombinant DNA
  • leader peptide
  • pBR339 vector
  • protein processing

ASJC Scopus subject areas

  • Genetics

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