Abstract
Many diseases trigger morphological changes in affected tissue. Today, classical histology is still the "gold standard" used to study and describe those changes. Classical histology, however, is time consuming and requires chemical tissue manipulations that can result in significant tissue distortions. It is sometimes difficult to separate tissue-processing artifacts from changes caused by the disease process. We show that synchrotron X-ray phase-contrast micro-computed tomography (micro-CT) can be used to examine non-embedded, hydrated tissue at a resolution comparable to that obtained with classical histology. The data analysis from stacks of reconstructed micro-CT images is more flexible and faster than when using the classical, physically embedded sections that are by necessity fixed in a particular orientation. We show that in a three-dimensional (3D) structure with meticulous structural details such as the cochlea and the kidney, micro-CT is more flexible, faster and more convenient for morphological studies and disease diagnoses.
| Original language | English (US) |
|---|---|
| Title of host publication | Developments in X-Ray Tomography X |
| Editors | Ge Wang, Stuart R. Stock, Bert Muller |
| Publisher | SPIE |
| ISBN (Electronic) | 9781510603257 |
| DOIs | |
| State | Published - Jan 1 2016 |
| Event | Developments in X-Ray Tomography X - San Diego, United States Duration: Aug 29 2016 → Aug 31 2016 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 9967 |
| ISSN (Print) | 0277-786X |
| ISSN (Electronic) | 1996-756X |
Other
| Other | Developments in X-Ray Tomography X |
|---|---|
| Country | United States |
| City | San Diego |
| Period | 8/29/16 → 8/31/16 |
Fingerprint
Keywords
- X-rays
- cochlea
- histology
- hyaline cartilage
- kidney
- phase contrast
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering
Cite this
}
A comparison of classical histology to anatomy revealed by hard X-rays. / Richter, Claus-Peter; Tan, Xiaodong; Young, Hunter; Stock, Stuart R; Robinson, Alan; Byskosh, Orest; Zheng, Jing; Soriano, Carmen; Xiao, Xianghui; Whitlon, Donna S.
Developments in X-Ray Tomography X. ed. / Ge Wang; Stuart R. Stock; Bert Muller. SPIE, 2016. 99671I (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9967).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - A comparison of classical histology to anatomy revealed by hard X-rays
AU - Richter, Claus-Peter
AU - Tan, Xiaodong
AU - Young, Hunter
AU - Stock, Stuart R
AU - Robinson, Alan
AU - Byskosh, Orest
AU - Zheng, Jing
AU - Soriano, Carmen
AU - Xiao, Xianghui
AU - Whitlon, Donna S
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Many diseases trigger morphological changes in affected tissue. Today, classical histology is still the "gold standard" used to study and describe those changes. Classical histology, however, is time consuming and requires chemical tissue manipulations that can result in significant tissue distortions. It is sometimes difficult to separate tissue-processing artifacts from changes caused by the disease process. We show that synchrotron X-ray phase-contrast micro-computed tomography (micro-CT) can be used to examine non-embedded, hydrated tissue at a resolution comparable to that obtained with classical histology. The data analysis from stacks of reconstructed micro-CT images is more flexible and faster than when using the classical, physically embedded sections that are by necessity fixed in a particular orientation. We show that in a three-dimensional (3D) structure with meticulous structural details such as the cochlea and the kidney, micro-CT is more flexible, faster and more convenient for morphological studies and disease diagnoses.
AB - Many diseases trigger morphological changes in affected tissue. Today, classical histology is still the "gold standard" used to study and describe those changes. Classical histology, however, is time consuming and requires chemical tissue manipulations that can result in significant tissue distortions. It is sometimes difficult to separate tissue-processing artifacts from changes caused by the disease process. We show that synchrotron X-ray phase-contrast micro-computed tomography (micro-CT) can be used to examine non-embedded, hydrated tissue at a resolution comparable to that obtained with classical histology. The data analysis from stacks of reconstructed micro-CT images is more flexible and faster than when using the classical, physically embedded sections that are by necessity fixed in a particular orientation. We show that in a three-dimensional (3D) structure with meticulous structural details such as the cochlea and the kidney, micro-CT is more flexible, faster and more convenient for morphological studies and disease diagnoses.
KW - X-rays
KW - cochlea
KW - histology
KW - hyaline cartilage
KW - kidney
KW - phase contrast
UR - http://www.scopus.com/inward/record.url?scp=85007079710&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007079710&partnerID=8YFLogxK
U2 - 10.1117/12.2239146
DO - 10.1117/12.2239146
M3 - Conference contribution
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Developments in X-Ray Tomography X
A2 - Wang, Ge
A2 - Stock, Stuart R.
A2 - Muller, Bert
PB - SPIE
ER -