@inproceedings{6e185e606e12477eb94749b397186d7c,
title = "A comparison of classical histology to anatomy revealed by hard X-rays",
abstract = "Many diseases trigger morphological changes in affected tissue. Today, classical histology is still the {"}gold standard{"} used to study and describe those changes. Classical histology, however, is time consuming and requires chemical tissue manipulations that can result in significant tissue distortions. It is sometimes difficult to separate tissue-processing artifacts from changes caused by the disease process. We show that synchrotron X-ray phase-contrast micro-computed tomography (micro-CT) can be used to examine non-embedded, hydrated tissue at a resolution comparable to that obtained with classical histology. The data analysis from stacks of reconstructed micro-CT images is more flexible and faster than when using the classical, physically embedded sections that are by necessity fixed in a particular orientation. We show that in a three-dimensional (3D) structure with meticulous structural details such as the cochlea and the kidney, micro-CT is more flexible, faster and more convenient for morphological studies and disease diagnoses.",
keywords = "X-rays, cochlea, histology, hyaline cartilage, kidney, phase contrast",
author = "Claus-Peter Richter and Xiaodong Tan and Hunter Young and Stock, {Stuart R} and Alan Robinson and Orest Byskosh and Jing Zheng and Carmen Soriano and Xianghui Xiao and Whitlon, {Donna S}",
note = "Funding Information: The APS is supported by the U.S. DOE, Basic Energy Sciences, Office of Science under contract No. W-31-109-ENG-38. The work is also supported by a grant from the Office of Naval Research (N00014-12-1-0173) and is supported in part with federal funds from the NIDCD, R01-DC011855 to CPR, R01-DC011813 to JZ, and ONR N00014-15-1-2130 to DSW. Publisher Copyright: {\textcopyright} Copyright 2016 SPIE.; Developments in X-Ray Tomography X ; Conference date: 29-08-2016 Through 31-08-2016",
year = "2016",
month = jan,
day = "1",
doi = "10.1117/12.2239146",
language = "English (US)",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Ge Wang and Stock, {Stuart R.} and Bert Muller",
booktitle = "Developments in X-Ray Tomography X",
}