A comparison of cutaneous testing and ELISA testing for assessing reactivity to ethylene oxide-human serum albumin in hemodialysis patients with anaphylactic reactions

Leslie C Grammer III*, Mary Roberts, Carol A. Wiggins, Richard R. Fitzsimons, Peter T Ivanovich, David M. Roxe, Roy Patterson

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

In five patients who have experienced anaphylaxis and in 29 patients who have not had such episodes during hemodialysis, we have performed two immunologic studies: cutaneous testing with ethylene oxide-human serum albumin (ETO-HSA) and ELISA for IgE against ETO-HSA. Four of five patients with reactions had positive cutaneous tests, whereas only one nonreactor had a positive skin test (p < 0.0002). The same four of five patients with reactions also had positive ELISA results, whereas three nonreactors has positive ELISA results (p < 0.003). In this group of patients, the positive predictive value of cutaneous testing (80%) is somewhat higher than that of ELISA testing (57%). However, the sensitivity, specificity, and negative predictive values are similar. We conclude that cutaneous testing with ETO-HSA probably offers a small advantage over IgE against ETO-HSA as determined by ELISA.

Original languageEnglish (US)
Pages (from-to)674-676
Number of pages3
JournalThe Journal of Allergy and Clinical Immunology
Volume87
Issue number3
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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