A comparison of levels of intrinsic single strand breaks/alkali labile sites associated with human melanoma cell invasion

L. C. Meade-Tollin*, B. L. Pipes, S. J. Anderson, E. A. Seftor, M. J.C. Hendrix

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Intrinsic levels of protein-free single strand breaks/alkali-labile sites in human melanoma cell populations of varying in vitro invasive capacity have been assayed with DNA filter elution methodology. DNA from two human melanoma cell lines, A375P and C8161, and from a subpopulation selected from A375P, A375P-5, were assayed to test the hypothesis that increased levels of DNA damage may be associated with the phenotype of increased invasive and metastatic capacities. The elution profiles obtained reveal statistically significant increases in the level of single strand breaks and/or alkali-labile sites ( SSB ALS) which correlate with increasing invasive and metastatic capacities. The increased levels of SSB ALS in A375P-5 observed in freshly selected cells decline as these cells are maintained in culture. The stability of this A375P-5 phenotype correlates with previously reported levels of double minute chromosomes, an indicator of genomic instability. Alterations in average intrinsic levels of cellular lesions are therefore an additional factor to be considered in the phenotypic characterization of invasive and metastatic tumor cells and may reflect or contribute to the genomic instability characteristic of tumor cell populations.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalCancer Letters
Volume53
Issue number1
DOIs
StatePublished - Aug 1990

Keywords

  • DNA damage
  • alkaline elution
  • invasion
  • melanoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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