A CRISPR screen identifies a pathway required for paraquat-induced cell death

Colleen R. Reczek, Kivanc Birsoy, Hyewon Kong, Inmaculada Martínez-Reyes, Tim Wang, Peng Gao, David M. Sabatini, Navdeep S. Chandel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Paraquat, a herbicide linked to Parkinson's disease, generates reactive oxygen species (ROS), which causes cell death. Because the source of paraquat-induced ROS production remains unknown, we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. Our screen uncovered three genes, POR (cytochrome P450 oxidoreductase), ATP7A (copper transporter), and SLC45A4 (sucrose transporter), required for paraquat-induced cell death. Furthermore, our results revealed POR as the source of paraquat-induced ROS production. Thus, our study highlights the use of functional genomic screens for uncovering redox biology.

Original languageEnglish (US)
Pages (from-to)1274-1279
Number of pages6
JournalNature Chemical Biology
Volume13
Issue number12
DOIs
StatePublished - Dec 1 2017

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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