TY - JOUR
T1 - A Cross-Linking Approach to Stabilizing Stimuli-Responsive Colloidal Crystals Engineered with DNA
AU - Lee, Seungkyu
AU - Zheng, Cindy Y.
AU - Bujold, Katherine E.
AU - Mirkin, Chad A.
N1 - Funding Information:
This material is based upon work supported by the Air Force Office of Scientific Research under Award FA9550-17-1-0348 and the Vannevar Bush Faculty Fellowship program sponsored by the Basic Research Office of the Assistant Secretary of Defense for Research and Engineering and funded by the Office of Naval Research through grant N00014-15-1-0043. This work made use of the EPIC facility of Northwestern University’s NUANCE Center, which receives support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205); the MRSEC program (NSFDMR-1121262) at the Materials Research Center; the International Institute for Nanotechnology (IIN) and the State of Illinois, through the IIN. Use of the Dupont–Northwestern–Dow Collaborative Access Team beamline at the Advanced Photon Source (APS) in Argonne National Laboratory was supported by the U.S. Department of Energy (DE-AC02-06CH11357). K.E.B. is supported by a Banting Postdoctoral Fellowship from the Natural Sciences and Engineering Council of Canada.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/7/31
Y1 - 2019/7/31
N2 - Two DNA-cross-linking reagents, bis-chloroethylnitrosourea and 8-methoxypsoralen, are used to covalently cross-link interstrand base pairs in DNA bonds that, in part, define colloidal crystals engineered with DNA. The irreversible linkages formed increase the chemical and thermal stability of the crystals and do not significantly affect their long-range order, as evidenced by small-angle X-ray scattering data. The post-modified crystals are stable in environments that the pre-modified structures are not, including solvents that encompass a broad range of polarities from ethanol to hexanes, and in aqueous media at pH 0 and 14. Interestingly, the cross-linked DNA bonds within these crystals still retain their flexibility, which is reflected by a solvent-dependent reversible change in lattice parameter. Since these organic cross-linking reagents, in comparison with inorganic approaches (use of silver ions or SiO2), have marginal effects on the composition and properties of the crystals, they provide an attractive alternative for stabilizing colloidal crystals engineered with DNA and make them potentially useful in a broader range of media.
AB - Two DNA-cross-linking reagents, bis-chloroethylnitrosourea and 8-methoxypsoralen, are used to covalently cross-link interstrand base pairs in DNA bonds that, in part, define colloidal crystals engineered with DNA. The irreversible linkages formed increase the chemical and thermal stability of the crystals and do not significantly affect their long-range order, as evidenced by small-angle X-ray scattering data. The post-modified crystals are stable in environments that the pre-modified structures are not, including solvents that encompass a broad range of polarities from ethanol to hexanes, and in aqueous media at pH 0 and 14. Interestingly, the cross-linked DNA bonds within these crystals still retain their flexibility, which is reflected by a solvent-dependent reversible change in lattice parameter. Since these organic cross-linking reagents, in comparison with inorganic approaches (use of silver ions or SiO2), have marginal effects on the composition and properties of the crystals, they provide an attractive alternative for stabilizing colloidal crystals engineered with DNA and make them potentially useful in a broader range of media.
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U2 - 10.1021/jacs.9b06106
DO - 10.1021/jacs.9b06106
M3 - Article
C2 - 31318543
AN - SCOPUS:85070786894
SN - 0002-7863
VL - 141
SP - 11827
EP - 11831
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 30
ER -