A distinct pathway of cell-mediated apoptosis initiated by Granulysin

A. A. Kaspar; S. Okada; J. Kumar; F. R. Poulain; K. A. Drouvalakis; A. Kelekar; D. A. Hanson; R. M. Kluck; Y. Hitoshi; D. E. Johnson; C. J. Froelich; C. B. Thompson; D. D. Newmeyer; A. Anel; C. Clayberger; A. M. Krensky

doi:10.4049/jimmunol.167.1.350

A distinct pathway of cell-mediated apoptosis initiated by Granulysin

A. A. Kaspar, S. Okada, J. Kumar, F. R. Poulain, K. A. Drouvalakis, A. Kelekar, D. A. Hanson, R. M. Kluck, Y. Hitoshi, D. E. Johnson, C. J. Froelich, C. B. Thompson, D. D. Newmeyer, A. Anel, C. Clayberger, A. M. Krensky^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Δψ) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.

Original language	English (US)
Pages (from-to)	350-356
Number of pages	7
Journal	Journal of Immunology
Volume	167
Issue number	1
DOIs	https://doi.org/10.4049/jimmunol.167.1.350
State	Published - Jul 1 2001

Funding

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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Kaspar, A. A., Okada, S., Kumar, J., Poulain, F. R., Drouvalakis, K. A., Kelekar, A., Hanson, D. A., Kluck, R. M., Hitoshi, Y., Johnson, D. E., Froelich, C. J., Thompson, C. B., Newmeyer, D. D., Anel, A., Clayberger, C., & Krensky, A. M. (2001). A distinct pathway of cell-mediated apoptosis initiated by Granulysin. Journal of Immunology, 167(1), 350-356. https://doi.org/10.4049/jimmunol.167.1.350

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abstract = "Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Δψ) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.",

author = "Kaspar, {A. A.} and S. Okada and J. Kumar and Poulain, {F. R.} and Drouvalakis, {K. A.} and A. Kelekar and Hanson, {D. A.} and Kluck, {R. M.} and Y. Hitoshi and Johnson, {D. E.} and Froelich, {C. J.} and Thompson, {C. B.} and Newmeyer, {D. D.} and A. Anel and C. Clayberger and Krensky, {A. M.}",

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Kaspar, AA, Okada, S, Kumar, J, Poulain, FR, Drouvalakis, KA, Kelekar, A, Hanson, DA, Kluck, RM, Hitoshi, Y, Johnson, DE, Froelich, CJ, Thompson, CB, Newmeyer, DD, Anel, A, Clayberger, C & Krensky, AM 2001, 'A distinct pathway of cell-mediated apoptosis initiated by Granulysin', Journal of Immunology, vol. 167, no. 1, pp. 350-356. https://doi.org/10.4049/jimmunol.167.1.350

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AU - Kaspar, A. A.

AU - Okada, S.

AU - Kumar, J.

AU - Poulain, F. R.

AU - Drouvalakis, K. A.

AU - Kelekar, A.

AU - Hanson, D. A.

AU - Kluck, R. M.

AU - Hitoshi, Y.

AU - Johnson, D. E.

AU - Froelich, C. J.

AU - Thompson, C. B.

AU - Newmeyer, D. D.

AU - Anel, A.

AU - Clayberger, C.

AU - Krensky, A. M.

PY - 2001/7/1

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AB - Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Δψ) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.

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A distinct pathway of cell-mediated apoptosis initiated by Granulysin

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