Abstract
There is a constant drive for affordable point-of-care testing (POCT) technologies for the detection of infectious human diseases. Herein, we report a simple platform for DNA detection that takes advantage of four techniques: commercially available pregnancy test strips (PTS), amplicon generation via loop-mediated isothermal amplification (LAMP), toehold-mediated strand displacement, and noncovalent immobilization of DNA on paper surface with DNA nanoflowers. This simple, separation-free platform is highly specific, as demonstrated with the detection of rtL180M, a single-nucleotide polymorphism observed in hepatitis B virus (HBV) associated with antiviral drug resistance. It is very sensitive, capable of detecting the targeted mutation at 2 copies μL−1. It is able to correctly identify the unmutated and rtL180M genome types of HBV in clinical samples. Given its wide adaptability, we expect this platform can be easily modified for the detection of genetic variations associated with various pathogens and human diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 24823-24827 |
| Number of pages | 5 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 60 |
| Issue number | 47 |
| DOIs | |
| State | Published - Nov 15 2021 |
Funding
This work was supported by the National Natural Science Foundation of China (Nos. 21874129 and 22174137), the International Scientific Cooperation Project of Jilin Scientific and Technological Development Program (No. 20200801044GH), and the Natural Sciences and Engineering Research Council of Canada (NSERC; RGPIN‐2020‐06401).
Keywords
- DNA detection
- DNA nanoflowers
- loop-mediated isothermal amplification
- pregnancy test strips
- toehold-mediated strand displacement
ASJC Scopus subject areas
- Catalysis
- General Chemistry
