A double-antibody radioimmunoassay for specific IgE to ragweed antigen E

Leslie C. Grammer; C. R. Zeiss; S. G. Hendrix; J. J. Pruzansky

A double-antibody radioimmunoassay for specific IgE to ragweed antigen E

Leslie C. Grammer, C. R. Zeiss, S. G. Hendrix, J. J. Pruzansky^*

^*Corresponding author for this work

Medicine, Allergy Division

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A double-antibody radioimmunoassay has been developed for quantitating IgE-a-AgE in serum. The patient's IgE is complexed by rabbit anti-human IgE, which is then precipitated with GARG. Radiolabeled AgE is then added to the washed precipitate and is specifically bound by IgE-a-AgE. This assay is highly reproducible, and unlike specific IgE assays based on the RAST concept, it cannot be inhibited by specific antibody of other immunoglobulin classes (blocking antibody). In addition, the double-antibody method does not require the use of myeloma IgE, and its results correlate well with those of a previously developed polystyrene tube method.

Original language	English (US)
Pages (from-to)	181-188
Number of pages	8
Journal	The Journal of laboratory and clinical medicine
Volume	98
Issue number	2
State	Published - Aug 1981

ASJC Scopus subject areas

Pathology and Forensic Medicine

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title = "A double-antibody radioimmunoassay for specific IgE to ragweed antigen E",

abstract = "A double-antibody radioimmunoassay has been developed for quantitating IgE-a-AgE in serum. The patient's IgE is complexed by rabbit anti-human IgE, which is then precipitated with GARG. Radiolabeled AgE is then added to the washed precipitate and is specifically bound by IgE-a-AgE. This assay is highly reproducible, and unlike specific IgE assays based on the RAST concept, it cannot be inhibited by specific antibody of other immunoglobulin classes (blocking antibody). In addition, the double-antibody method does not require the use of myeloma IgE, and its results correlate well with those of a previously developed polystyrene tube method.",

author = "Grammer, {Leslie C.} and Zeiss, {C. R.} and Hendrix, {S. G.} and Pruzansky, {J. J.}",

year = "1981",

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language = "English (US)",

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AU - Grammer, Leslie C.

AU - Zeiss, C. R.

AU - Hendrix, S. G.

AU - Pruzansky, J. J.

PY - 1981/8

Y1 - 1981/8

N2 - A double-antibody radioimmunoassay has been developed for quantitating IgE-a-AgE in serum. The patient's IgE is complexed by rabbit anti-human IgE, which is then precipitated with GARG. Radiolabeled AgE is then added to the washed precipitate and is specifically bound by IgE-a-AgE. This assay is highly reproducible, and unlike specific IgE assays based on the RAST concept, it cannot be inhibited by specific antibody of other immunoglobulin classes (blocking antibody). In addition, the double-antibody method does not require the use of myeloma IgE, and its results correlate well with those of a previously developed polystyrene tube method.

AB - A double-antibody radioimmunoassay has been developed for quantitating IgE-a-AgE in serum. The patient's IgE is complexed by rabbit anti-human IgE, which is then precipitated with GARG. Radiolabeled AgE is then added to the washed precipitate and is specifically bound by IgE-a-AgE. This assay is highly reproducible, and unlike specific IgE assays based on the RAST concept, it cannot be inhibited by specific antibody of other immunoglobulin classes (blocking antibody). In addition, the double-antibody method does not require the use of myeloma IgE, and its results correlate well with those of a previously developed polystyrene tube method.

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