A Drosophila single-strand DNA/RNA-binding factor contains a high-mobility-group box and is enriched in the nucleolus

Tien Hsu, Dennis L. King, Carole Labonne, Fotis C. Kafatos*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

We have isolated a Drosophila melanogaster cDNA encoding a high-mobility-group (HMG) box-containing protein. This protein shares 50% amino acid identity with the human putative structure-specific recognition protein, hSSRP. The gene encoding the D. melanogaster homolog, DssRP, is developmentally regulated and is expressed most abundantly in ovaries (nurse cells in particular). The protein is localized in nuclei and is particularly abundant in the nucleolus. In vitro binding studies using DssRP produced in bacteria showed that, despite expectation, the protein does not bind to structured DNA. Instead, it binds to single-stranded DNA and RNA, with highest affinity to nucleotides G and U.

Original languageEnglish (US)
Pages (from-to)6488-6492
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number14
DOIs
StatePublished - Jul 15 1993

Keywords

  • Drosophila nucleolar factor
  • Single-strand binding factor

ASJC Scopus subject areas

  • General

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