A dynamic spatiotemporal extracellular matrix facilitates epicardial-mediated vertebrate heart regeneration

Sarah E. Mercer, Shannon J. Odelberg, Hans Georg Simon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

104 Scopus citations


Unlike humans, certain adult vertebrates such as newts and zebrafish possess extraordinary abilities to functionally regenerate lost appendages and injured organs, including cardiac muscle. Here, we present new evidence that a remodeled extracellular matrix (ECM) directs cell activities essential for cardiac muscle regeneration. Comprehensive mining of DNA microarrays and Gene Ontology term enrichment analyses for regenerating newt and zebrafish hearts revealed that distinct ECM components and ECM-modifying proteases are among the most significantly enriched genes in response to local injury. In contrast, data analyses for mammalian cardiac injury models indicated that inflammation and metabolic processes are the most significantly activated gene groups. In the regenerating newt heart, we show dynamic spatial and temporal changes in tenascin-C, hyaluronic acid, and fibronectin ECM distribution as early as 3 days postamputation. Linked to distinct matrix remodeling, we demonstrate a myocardium-wide proliferative response and radial migration of progenitor cells. In particular, we report dramatic upregulation of a regeneration-specific matrix in the epicardium that precedes the accumulation and migration of progenitor cells. For the first time, we show that the regenerative ECM component tenascin-C significantly increases newt cardiomyocyte cell cycle reentry in vitro. Thus, the engineering of nature-tested extracellular matrices may provide new strategic opportunities for the enhancement of regenerative responses in mammals.

Original languageEnglish (US)
Pages (from-to)457-469
Number of pages13
JournalDevelopmental Biology
Issue number2
StatePublished - Oct 15 2013


  • Extracellular matrix
  • Heart
  • Myocardial infarction
  • Newt
  • Regeneration

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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