A fluorophore-based bio-barcode amplification assay for proteins

Byung Keun Oh*, Jwa Min Nam, Seung Woo Lee, Chad A. Mirkin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

A novel type of bio-barcode amplification assay that relies on the in situ detection of fluorophore-labeled barcodes attached to polystyrene microparticles was investigated. It was found that the new assay required only 90 mins to be carried out and without the need for enzymatic amplification or microarray analysis. The fluorophore-based bio-barcode amplification method simplifies the detection process by eliminating the array detection and barcode sorting method. The results show that the bio-barcode method offered a degree of detection simplicity when only a few targets are to be identified in a test sample.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalSmall
Volume2
Issue number1
DOIs
StatePublished - Jan 2006

Keywords

  • Diagnostics
  • Fluorescent probes
  • Magnetic materials
  • Microstructures
  • Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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