Abstract
This work uses global gene expression analysis to compare the extent to which model substrates presenting peptide adhesion motifs mimic the use of conventional extracellular matrix protein coated substrates for cell culture. We compared the transcriptional activities of genes in cells that were cultured on matrix-coated substrates with those cultured on self-assembled monolayers presenting either a linear or cyclic RGD peptide. Cells adherent to cyclic RGD were most similar to those cultured on native ECM, while cells cultured on monolayers presenting the linear RGD peptide had transcriptional activities that were more similar to cells cultured on the uncoated substrates. This study suggests that biomaterials presenting the cyclic RGD peptide are substantially better mimics of extracellular matrix than are uncoated materials or materials presenting the common linear RGD peptide.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 385-394 |
| Number of pages | 10 |
| Journal | Biomaterials |
| Volume | 52 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2015 |
Funding
This research was supported by the Center of Cancer Nanotechnology Excellence (CCNE) initiative of the National Institutes of Health (NIH) under Award number U54 CA151880 and the Zell Family Foundation Faculty Recruitment Gift. Microarray expression profiling was performed by the Northwestern University Genomics Core Facility. Expression analysis was performed by the Northwestern University Bioinformatics Core.
Keywords
- Biomimetic material
- Fibronectin
- Integrin
- Self-assembled monolayer
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Ceramics and Composites
- Biomaterials
- Mechanics of Materials