A gene expression-based comparison of cell adhesion to extracellular matrix and RGD-terminated monolayers

Courtney J. Sobers, Sarah E. Wood, Milan Mrksich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

This work uses global gene expression analysis to compare the extent to which model substrates presenting peptide adhesion motifs mimic the use of conventional extracellular matrix protein coated substrates for cell culture. We compared the transcriptional activities of genes in cells that were cultured on matrix-coated substrates with those cultured on self-assembled monolayers presenting either a linear or cyclic RGD peptide. Cells adherent to cyclic RGD were most similar to those cultured on native ECM, while cells cultured on monolayers presenting the linear RGD peptide had transcriptional activities that were more similar to cells cultured on the uncoated substrates. This study suggests that biomaterials presenting the cyclic RGD peptide are substantially better mimics of extracellular matrix than are uncoated materials or materials presenting the common linear RGD peptide.

Original languageEnglish (US)
Pages (from-to)385-394
Number of pages10
JournalBiomaterials
Volume52
Issue number1
DOIs
StatePublished - 2015

Keywords

  • Biomimetic material
  • Fibronectin
  • Integrin
  • Self-assembled monolayer

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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