A genome-wide association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A

Jérôme Lane; Paul J. Mclaren; Lucy Dorrell; Kevin V. Shianna; Amanda Stemke; Kimberly Pelak; Stephen Moore; Johannes Oldenburg; Maria Teresa Alvarez-Roman; Anne Angelillo-Scherrer; Francoise Boehlen; Paula H.B. Bolton-Maggs; Brigit Brand; Deborah Brown; Elaine Chiang; Ana Rosa Cid-Haro; Bonaventura Clotet; Peter Collins; Sara Colombo; Judith Dalmau; Patrick Fogarty; Paul Giangrande; Alessandro Gringeri; Rathi Iyer; Olga Katsarou; Christine Kempton; Philip Kuriakose; Judith Lin; Mike Makris; Marilyn Manco-Johnson; Dimitrios A. Tsakiris; Javier Martinez-Picado; Evelien Mauser-Bunschoten; Anne Neff; Shinichi Oka; Lara Oyesiku; Rafael Parra; Kristiina Peter-Salonen; Jerry Powell; Michael Recht; Amy Shapiro; Kimo Stine; Katherine Talks; Amalio Telenti; Jonathan Wilde; Thynn Thynn Yee; Steven M. Wolinsky; Jeremy Martinson; Shehnaz K. Hussain; Jay H. Bream; Lisa P. Jacobson; Mary Carrington; James J. Goedert; Barton F. Haynes; Andrew J. Mcmichael; David B. Goldstein; Jacques Fellay

doi:10.1093/hmg/ddt033

A genome-wide association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A

Jérôme Lane, Paul J. Mclaren, Lucy Dorrell, Kevin V. Shianna, Amanda Stemke, Kimberly Pelak, Stephen Moore, Johannes Oldenburg, Maria Teresa Alvarez-Roman, Anne Angelillo-Scherrer, Francoise Boehlen, Paula H.B. Bolton-Maggs, Brigit Brand, Deborah Brown, Elaine Chiang, Ana Rosa Cid-Haro, Bonaventura Clotet, Peter Collins, Sara Colombo, Judith DalmauPatrick Fogarty, Paul Giangrande, Alessandro Gringeri, Rathi Iyer, Olga Katsarou, Christine Kempton, Philip Kuriakose, Judith Lin, Mike Makris, Marilyn Manco-Johnson, Dimitrios A. Tsakiris, Javier Martinez-Picado, Evelien Mauser-Bunschoten, Anne Neff, Shinichi Oka, Lara Oyesiku, Rafael Parra, Kristiina Peter-Salonen, Jerry Powell, Michael Recht, Amy Shapiro, Kimo Stine, Katherine Talks, Amalio Telenti, Jonathan Wilde, Thynn Thynn Yee, Steven M. Wolinsky, Jeremy Martinson, Shehnaz K. Hussain, Jay H. Bream, Lisa P. Jacobson, Mary Carrington, James J. Goedert, Barton F. Haynes, Andrew J. Mcmichael, David B. Goldstein, Jacques Fellay^*

^*Corresponding author for this work

Medicine, Infectious Diseases Division

Research output: Contribution to journal › Article › peer-review

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Abstract

Human genetic variation contributes to differences in susceptibility to HIV-1 infection. To search for novel host resistance factors, we performed a genome-wide association study (GWAS) in hemophilia patients highly exposed to potentially contaminated factor VIII infusions.Individuals with hemophilia A and a documented history of factor VIII infusions before the introduction of viral inactivation procedures (1979-1984) were recruited from 36 hemophilia treatment centers (HTCs), and their genome-wide genetic variants were compared with those from matched HIV-infected individuals. Homozygous carriers of known CCR5 resistance mutations were excluded. Single nucleotide polymorphisms (SNPs) and inferred copy number variants (CNVs) were tested using logistic regression. In addition, we performed a pathway enrichment analysis, a heritability analysis, and a search for epistatic interactions with CCR5 δ32 heterozygosity.A total of 560 HIV-uninfected cases were recruited: 36 (6.4%) were homozygous for CCR5 δ32 or m303. After quality control and SNP imputation, we tested 1 081 435 SNPs and 3686 CNVs for association with HIV-1 serostatus in 431 cases and 765 HIV-infected controls. No SNP or CNV reached genome-wide significance. The additional analyses did not reveal any strong genetic effect.Highly exposed, yet uninfected hemophiliacs form an ideal study group to investigate host resistance factors. Using a genome-wide approach, we did not detect any significant associations between SNPs and HIV-1 susceptibility, indicating that common genetic variants of major effect are unlikely to explain the observed resistance phenotype in this population.

Original language	English (US)
Article number	ddt033
Pages (from-to)	1903-1910
Number of pages	8
Journal	Human molecular genetics
Volume	22
Issue number	9
DOIs	https://doi.org/10.1093/hmg/ddt033
State	Published - May 2013

Funding

This work was supported by the National Institute of Allergy and Infectious Diseases Center for HIV/AIDS Vaccine Immunology (CHAVI) (AI067854). This project has been funded in part with federal funds from the Frederick National Laboratory for Cancer Research (HHSN261200800001E). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products or organizations imply endorsement by the US Government. This research was supported in part by the Intramural Research Program of the National Institutes of Health, Frederick National Lab, Center for Cancer Research. The Multicenter AIDS Cohort Study is funded by the National Institute of Allergy and Infectious Diseases, with additional supplemental funding from the National Cancer Institute (UO1-AI-35042, UL1-RR025005, UO1-AI-35043, UO1-AI-35039, UO1-AI-35040, UO1-AI-35041).

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

UN SDGs

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10.1093/hmg/ddt033

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Lane, J., Mclaren, P. J., Dorrell, L., Shianna, K. V., Stemke, A., Pelak, K., Moore, S., Oldenburg, J., Alvarez-Roman, M. T., Angelillo-Scherrer, A., Boehlen, F., Bolton-Maggs, P. H. B., Brand, B., Brown, D., Chiang, E., Cid-Haro, A. R., Clotet, B., Collins, P., Colombo, S., ... Fellay, J. (2013). A genome-wide association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A. Human molecular genetics, 22(9), 1903-1910. Article ddt033. https://doi.org/10.1093/hmg/ddt033

@article{6b3a3fea055e479b92fb5c51a59ee398,

title = "A genome-wide association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A",

abstract = "Human genetic variation contributes to differences in susceptibility to HIV-1 infection. To search for novel host resistance factors, we performed a genome-wide association study (GWAS) in hemophilia patients highly exposed to potentially contaminated factor VIII infusions.Individuals with hemophilia A and a documented history of factor VIII infusions before the introduction of viral inactivation procedures (1979-1984) were recruited from 36 hemophilia treatment centers (HTCs), and their genome-wide genetic variants were compared with those from matched HIV-infected individuals. Homozygous carriers of known CCR5 resistance mutations were excluded. Single nucleotide polymorphisms (SNPs) and inferred copy number variants (CNVs) were tested using logistic regression. In addition, we performed a pathway enrichment analysis, a heritability analysis, and a search for epistatic interactions with CCR5 δ32 heterozygosity.A total of 560 HIV-uninfected cases were recruited: 36 (6.4%) were homozygous for CCR5 δ32 or m303. After quality control and SNP imputation, we tested 1 081 435 SNPs and 3686 CNVs for association with HIV-1 serostatus in 431 cases and 765 HIV-infected controls. No SNP or CNV reached genome-wide significance. The additional analyses did not reveal any strong genetic effect.Highly exposed, yet uninfected hemophiliacs form an ideal study group to investigate host resistance factors. Using a genome-wide approach, we did not detect any significant associations between SNPs and HIV-1 susceptibility, indicating that common genetic variants of major effect are unlikely to explain the observed resistance phenotype in this population.",

author = "J{\'e}r{\^o}me Lane and Mclaren, {Paul J.} and Lucy Dorrell and Shianna, {Kevin V.} and Amanda Stemke and Kimberly Pelak and Stephen Moore and Johannes Oldenburg and Alvarez-Roman, {Maria Teresa} and Anne Angelillo-Scherrer and Francoise Boehlen and Bolton-Maggs, {Paula H.B.} and Brigit Brand and Deborah Brown and Elaine Chiang and Cid-Haro, {Ana Rosa} and Bonaventura Clotet and Peter Collins and Sara Colombo and Judith Dalmau and Patrick Fogarty and Paul Giangrande and Alessandro Gringeri and Rathi Iyer and Olga Katsarou and Christine Kempton and Philip Kuriakose and Judith Lin and Mike Makris and Marilyn Manco-Johnson and Tsakiris, {Dimitrios A.} and Javier Martinez-Picado and Evelien Mauser-Bunschoten and Anne Neff and Shinichi Oka and Lara Oyesiku and Rafael Parra and Kristiina Peter-Salonen and Jerry Powell and Michael Recht and Amy Shapiro and Kimo Stine and Katherine Talks and Amalio Telenti and Jonathan Wilde and Yee, {Thynn Thynn} and Wolinsky, {Steven M.} and Jeremy Martinson and Hussain, {Shehnaz K.} and Bream, {Jay H.} and Jacobson, {Lisa P.} and Mary Carrington and Goedert, {James J.} and Haynes, {Barton F.} and Mcmichael, {Andrew J.} and Goldstein, {David B.} and Jacques Fellay",

note = "Funding Information: This work was supported by the National Institute of Allergy and Infectious Diseases Center for HIV/AIDS Vaccine Immunology (CHAVI) (AI067854). This project has been funded in part with federal funds from the Frederick National Laboratory for Cancer Research (HHSN261200800001E). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products or organizations imply endorsement by the US Government. This research was supported in part by the Intramural Research Program of the National Institutes of Health, Frederick National Lab, Center for Cancer Research. The Multicenter AIDS Cohort Study is funded by the National Institute of Allergy and Infectious Diseases, with additional supplemental funding from the National Cancer Institute (UO1-AI-35042, UL1-RR025005, UO1-AI-35043, UO1-AI-35039, UO1-AI-35040, UO1-AI-35041).",

year = "2013",

month = may,

doi = "10.1093/hmg/ddt033",

language = "English (US)",

volume = "22",

pages = "1903--1910",

journal = "Human molecular genetics",

issn = "0964-6906",

publisher = "Oxford University Press",

number = "9",

}

Lane, J, Mclaren, PJ, Dorrell, L, Shianna, KV, Stemke, A, Pelak, K, Moore, S, Oldenburg, J, Alvarez-Roman, MT, Angelillo-Scherrer, A, Boehlen, F, Bolton-Maggs, PHB, Brand, B, Brown, D, Chiang, E, Cid-Haro, AR, Clotet, B, Collins, P, Colombo, S, Dalmau, J, Fogarty, P, Giangrande, P, Gringeri, A, Iyer, R, Katsarou, O, Kempton, C, Kuriakose, P, Lin, J, Makris, M, Manco-Johnson, M, Tsakiris, DA, Martinez-Picado, J, Mauser-Bunschoten, E, Neff, A, Oka, S, Oyesiku, L, Parra, R, Peter-Salonen, K, Powell, J, Recht, M, Shapiro, A, Stine, K, Talks, K, Telenti, A, Wilde, J, Yee, TT, Wolinsky, SM, Martinson, J, Hussain, SK, Bream, JH, Jacobson, LP, Carrington, M, Goedert, JJ, Haynes, BF, Mcmichael, AJ, Goldstein, DB & Fellay, J 2013, 'A genome-wide association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A', Human molecular genetics, vol. 22, no. 9, ddt033, pp. 1903-1910. https://doi.org/10.1093/hmg/ddt033

TY - JOUR

T1 - A genome-wide association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A

AU - Lane, Jérôme

AU - Mclaren, Paul J.

AU - Dorrell, Lucy

AU - Shianna, Kevin V.

AU - Stemke, Amanda

AU - Pelak, Kimberly

AU - Moore, Stephen

AU - Oldenburg, Johannes

AU - Alvarez-Roman, Maria Teresa

AU - Angelillo-Scherrer, Anne

AU - Boehlen, Francoise

AU - Bolton-Maggs, Paula H.B.

AU - Brand, Brigit

AU - Brown, Deborah

AU - Chiang, Elaine

AU - Cid-Haro, Ana Rosa

AU - Clotet, Bonaventura

AU - Collins, Peter

AU - Colombo, Sara

AU - Dalmau, Judith

AU - Fogarty, Patrick

AU - Giangrande, Paul

AU - Gringeri, Alessandro

AU - Iyer, Rathi

AU - Katsarou, Olga

AU - Kempton, Christine

AU - Kuriakose, Philip

AU - Lin, Judith

AU - Makris, Mike

AU - Manco-Johnson, Marilyn

AU - Tsakiris, Dimitrios A.

AU - Martinez-Picado, Javier

AU - Mauser-Bunschoten, Evelien

AU - Neff, Anne

AU - Oka, Shinichi

AU - Oyesiku, Lara

AU - Parra, Rafael

AU - Peter-Salonen, Kristiina

AU - Powell, Jerry

AU - Recht, Michael

AU - Shapiro, Amy

AU - Stine, Kimo

AU - Talks, Katherine

AU - Telenti, Amalio

AU - Wilde, Jonathan

AU - Yee, Thynn Thynn

AU - Wolinsky, Steven M.

AU - Martinson, Jeremy

AU - Hussain, Shehnaz K.

AU - Bream, Jay H.

AU - Jacobson, Lisa P.

AU - Carrington, Mary

AU - Goedert, James J.

AU - Haynes, Barton F.

AU - Mcmichael, Andrew J.

AU - Goldstein, David B.

AU - Fellay, Jacques

N1 - Funding Information: This work was supported by the National Institute of Allergy and Infectious Diseases Center for HIV/AIDS Vaccine Immunology (CHAVI) (AI067854). This project has been funded in part with federal funds from the Frederick National Laboratory for Cancer Research (HHSN261200800001E). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products or organizations imply endorsement by the US Government. This research was supported in part by the Intramural Research Program of the National Institutes of Health, Frederick National Lab, Center for Cancer Research. The Multicenter AIDS Cohort Study is funded by the National Institute of Allergy and Infectious Diseases, with additional supplemental funding from the National Cancer Institute (UO1-AI-35042, UL1-RR025005, UO1-AI-35043, UO1-AI-35039, UO1-AI-35040, UO1-AI-35041).

PY - 2013/5

Y1 - 2013/5

N2 - Human genetic variation contributes to differences in susceptibility to HIV-1 infection. To search for novel host resistance factors, we performed a genome-wide association study (GWAS) in hemophilia patients highly exposed to potentially contaminated factor VIII infusions.Individuals with hemophilia A and a documented history of factor VIII infusions before the introduction of viral inactivation procedures (1979-1984) were recruited from 36 hemophilia treatment centers (HTCs), and their genome-wide genetic variants were compared with those from matched HIV-infected individuals. Homozygous carriers of known CCR5 resistance mutations were excluded. Single nucleotide polymorphisms (SNPs) and inferred copy number variants (CNVs) were tested using logistic regression. In addition, we performed a pathway enrichment analysis, a heritability analysis, and a search for epistatic interactions with CCR5 δ32 heterozygosity.A total of 560 HIV-uninfected cases were recruited: 36 (6.4%) were homozygous for CCR5 δ32 or m303. After quality control and SNP imputation, we tested 1 081 435 SNPs and 3686 CNVs for association with HIV-1 serostatus in 431 cases and 765 HIV-infected controls. No SNP or CNV reached genome-wide significance. The additional analyses did not reveal any strong genetic effect.Highly exposed, yet uninfected hemophiliacs form an ideal study group to investigate host resistance factors. Using a genome-wide approach, we did not detect any significant associations between SNPs and HIV-1 susceptibility, indicating that common genetic variants of major effect are unlikely to explain the observed resistance phenotype in this population.

AB - Human genetic variation contributes to differences in susceptibility to HIV-1 infection. To search for novel host resistance factors, we performed a genome-wide association study (GWAS) in hemophilia patients highly exposed to potentially contaminated factor VIII infusions.Individuals with hemophilia A and a documented history of factor VIII infusions before the introduction of viral inactivation procedures (1979-1984) were recruited from 36 hemophilia treatment centers (HTCs), and their genome-wide genetic variants were compared with those from matched HIV-infected individuals. Homozygous carriers of known CCR5 resistance mutations were excluded. Single nucleotide polymorphisms (SNPs) and inferred copy number variants (CNVs) were tested using logistic regression. In addition, we performed a pathway enrichment analysis, a heritability analysis, and a search for epistatic interactions with CCR5 δ32 heterozygosity.A total of 560 HIV-uninfected cases were recruited: 36 (6.4%) were homozygous for CCR5 δ32 or m303. After quality control and SNP imputation, we tested 1 081 435 SNPs and 3686 CNVs for association with HIV-1 serostatus in 431 cases and 765 HIV-infected controls. No SNP or CNV reached genome-wide significance. The additional analyses did not reveal any strong genetic effect.Highly exposed, yet uninfected hemophiliacs form an ideal study group to investigate host resistance factors. Using a genome-wide approach, we did not detect any significant associations between SNPs and HIV-1 susceptibility, indicating that common genetic variants of major effect are unlikely to explain the observed resistance phenotype in this population.

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U2 - 10.1093/hmg/ddt033

DO - 10.1093/hmg/ddt033

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C2 - 23372042

AN - SCOPUS:84875914649

SN - 0964-6906

VL - 22

SP - 1903

EP - 1910

JO - Human molecular genetics

JF - Human molecular genetics

IS - 9

M1 - ddt033

ER -

A genome-wide association study of resistance to HIV infection in highly exposed uninfected individuals with hemophilia A

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