@article{c6614c5a43874dfb8bf38c0098400f18,
title = "A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells",
abstract = "Long-distance intracellular transport of organelles, mRNA, and proteins ({"}cargo{"}) occurs along the microtubule cytoskeleton by the action of kinesin and dynein motor proteins, but the vast network of factors involved in regulating intracellular cargo transport are still unknown. We capitalize on the Drosophila melanogaster S2 model cell system to monitor lysosome transport along microtubule bundles, which require enzymatically active kinesin-1 motor protein for their formation. We use an automated tracking program and a naive Bayesian classifier for the multivariate motility data to analyze 15,683 gene phenotypes and find 98 proteins involved in regulating lysosome motility along microtubules and 48 involved in the formation of microtubule filled processes in S2 cells. We identify innate immunity genes, ion channels, and signaling proteins having a role in lysosome motility regulation and find an unexpected relationship between the dynein motor, Rab7a, and lysosome motility regulation.",
author = "Jolly, {Amber L.} and Luan, {Chi Hao} and Dusel, {Brendon E.} and Dunne, {Sara F.} and Michael Winding and Dixit, {Vishrut J.} and Chloe Robins and Saluk, {Jennifer L.} and Logan, {David J.} and Carpenter, {Anne E.} and Manu Sharma and Deborah Dean and Cohen, {Andrew R.} and Gelfand, {Vladimir I.}",
note = "Funding Information: We would like to thank Pradeep Sivakumar (Northwestern University, Evanston, IL), Mark Bray, and the Carpenter lab (Broad Institute, MA) for help with batch image data processing. The project described was supported in part by the H Foundation Cancer Research Fund and Robert H. Lurie Comprehensive Cancer Center. The content is solely the responsibility of the authors and does not necessarily represent the official views of the H Foundation and Robert H. Lurie Comprehensive Cancer Center. Additional funding was provided by a National Science Foundation Graduate Research Fellowship Program fellowship to A.L.J. and National Institute of General Medical Science/National Institutes of Health grants R01 GM052111 to V.I.G. and R01 GM089652 to A.E.C. Funding Information: We would like to thank Pradeep Sivakumar (Northwestern University, Evanston, IL), Mark Bray, and the Carpenter lab (Broad Institute, MA) for help with batch image data processing. The project described was supported in part by the H Foundation Cancer Research Fund and Robert H. Lurie Comprehensive Cancer Center. The content is solely the responsibility of the authors and does not necessarily represent the official views of the H Foundation and Robert H. Lurie Comprehensive Cancer Center. Additional funding was provided by a National Science Foundation Graduate Research Fellowship Program fellowship to A.L.J. and National Institute of General Medical Science/National Institutes of Health grants R01 GM052111 to V.I.G. and R01 GM089652 to A.E.C. Publisher Copyright: {\textcopyright} 2016 The Authors.",
year = "2016",
month = jan,
day = "26",
doi = "10.1016/j.celrep.2015.12.051",
language = "English (US)",
volume = "14",
pages = "611--620",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "3",
}