A gold-nanoparticle-based real-time colorimetric screening method for endonuclease activity and inhibition

Xiaoyang Xu*, Min Su Han, Chad A. Mirkin

*Corresponding author for this work

Research output: Contribution to journalArticle

229 Scopus citations

Abstract

(Figure Presented) On the small screen: Aggregates of gold nanopartides interconnected by DNA duplexes (see scheme, left) are bluish-purple. Cleavage of the duplexes by deoxyribonuclease I (DNase I) releases the nanoparticles (right), producing a bluish-purple-to-red color change. This color change can be used to monitor the activity (and inhibition) of the enzyme.

Original languageEnglish (US)
Pages (from-to)3468-3470
Number of pages3
JournalAngewandte Chemie - International Edition
Volume46
Issue number19
DOIs
StatePublished - Jun 29 2007

Keywords

  • Endonucleases
  • High-throughput screening
  • Inhibitors
  • Nanoparticles
  • Oligonucleotides

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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