A heterophilic adhesion mechanism for platelet/endothelial cell adhesion molecule 1 (CD31)

William A. Muller; Miriam E. Berman; Peter J. Newman; Horace M. DeLisser; Steven M. Albelda

A heterophilic adhesion mechanism for platelet/endothelial cell adhesion molecule 1 (CD31)

William A. Muller^*, Miriam E. Berman, Peter J. Newman, Horace M. DeLisser, Steven M. Albelda

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

Abstract

The molecular nature of cell adhesion mediated by platelet/endothelial cell adhesion molecule 1 (PECAM-1; CD31) was examined using stably transfected L cells in a PECAM-dependent aggregation assay. This adhesion was temperature sensitive and divalent cation dependent, with Mg²⁺ supporting aggregation to a greater degree than Ca²⁺. PECAM-dependent aggregation was heterophilic: PECAM-1 transfectants bound as readily to control-transfected L cells as to other PECAM-1 transfectants, demonstrating that a molecule endogenously expressed on the L cells serves as the ligand for PECAM in this system and presumably substitutes for the natural human ligand.

Original language	English (US)
Pages (from-to)	1401-1404
Number of pages	4
Journal	Journal of Experimental Medicine
Volume	175
Issue number	5
State	Published - May 1 1992

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Cite this

@article{d448d7376bfa434ab0f8c3a1b9d04ad4,

title = "A heterophilic adhesion mechanism for platelet/endothelial cell adhesion molecule 1 (CD31)",

abstract = "The molecular nature of cell adhesion mediated by platelet/endothelial cell adhesion molecule 1 (PECAM-1; CD31) was examined using stably transfected L cells in a PECAM-dependent aggregation assay. This adhesion was temperature sensitive and divalent cation dependent, with Mg2+ supporting aggregation to a greater degree than Ca2+. PECAM-dependent aggregation was heterophilic: PECAM-1 transfectants bound as readily to control-transfected L cells as to other PECAM-1 transfectants, demonstrating that a molecule endogenously expressed on the L cells serves as the ligand for PECAM in this system and presumably substitutes for the natural human ligand.",

author = "Muller, {William A.} and Berman, {Miriam E.} and Newman, {Peter J.} and DeLisser, {Horace M.} and Albelda, {Steven M.}",

year = "1992",

month = may,

day = "1",

language = "English (US)",

volume = "175",

pages = "1401--1404",

journal = "Journal of Experimental Medicine",

issn = "0022-1007",

publisher = "Rockefeller University Press",

number = "5",

}

TY - JOUR

T1 - A heterophilic adhesion mechanism for platelet/endothelial cell adhesion molecule 1 (CD31)

AU - Muller, William A.

AU - Berman, Miriam E.

AU - Newman, Peter J.

AU - DeLisser, Horace M.

AU - Albelda, Steven M.

PY - 1992/5/1

Y1 - 1992/5/1

N2 - The molecular nature of cell adhesion mediated by platelet/endothelial cell adhesion molecule 1 (PECAM-1; CD31) was examined using stably transfected L cells in a PECAM-dependent aggregation assay. This adhesion was temperature sensitive and divalent cation dependent, with Mg2+ supporting aggregation to a greater degree than Ca2+. PECAM-dependent aggregation was heterophilic: PECAM-1 transfectants bound as readily to control-transfected L cells as to other PECAM-1 transfectants, demonstrating that a molecule endogenously expressed on the L cells serves as the ligand for PECAM in this system and presumably substitutes for the natural human ligand.

AB - The molecular nature of cell adhesion mediated by platelet/endothelial cell adhesion molecule 1 (PECAM-1; CD31) was examined using stably transfected L cells in a PECAM-dependent aggregation assay. This adhesion was temperature sensitive and divalent cation dependent, with Mg2+ supporting aggregation to a greater degree than Ca2+. PECAM-dependent aggregation was heterophilic: PECAM-1 transfectants bound as readily to control-transfected L cells as to other PECAM-1 transfectants, demonstrating that a molecule endogenously expressed on the L cells serves as the ligand for PECAM in this system and presumably substitutes for the natural human ligand.

UR - http://www.scopus.com/inward/record.url?scp=0026514985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026514985&partnerID=8YFLogxK

M3 - Article

C2 - 1569405

AN - SCOPUS:0026514985

SN - 0022-1007

VL - 175

SP - 1401

EP - 1404

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

IS - 5

ER -

A heterophilic adhesion mechanism for platelet/endothelial cell adhesion molecule 1 (CD31)

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this