Abstract
The neural stem cell marker CD133 is reported to identify cells within glioblastoma (GBM) that can initiate neurosphere growth and tumor formation; however, instances of CD133- cells exhibiting similar properties have also been reported. Here, we show that some PTEN-deficient GBM tumors produce a series of CD133+ and CD133- self-renewing tumor-initiating cell types and provide evidence that these cell types constitute a lineage hierarchy. Our results show that the capacities for self-renewal and tumor initiation in GBM need not be restricted to a uniform population of stemlike cells, but can be shared by a lineage of self-renewing cell types expressing a range of markers of forebrain lineage.
Original language | English (US) |
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Pages (from-to) | 362-375 |
Number of pages | 14 |
Journal | Cancer cell |
Volume | 17 |
Issue number | 4 |
DOIs | |
State | Published - Apr 13 2010 |
Funding
We thank M. Baatz and J. Zimmermann for implementation of the image analysis solution; J. Eastham-Anderson and J. Zha for assistance with histological analysis; T. Le, W. Tombo, and J. Ayers for expert technical assistance; J. Cupp for FACS consultation; A. Bruce for graphics; T. Wu for bioinformatics support; and G. Plowman for helpful discussions. This study was supported by the State of California and the University of California Industry-University Cooperative Research Discovery (grant Bio05-10501), the National Brain Tumor Society (support to J.F.C. and S.M.), and by the National Institutes of Health (grant CA 097257 to C.D.J., S.R.V., and M.D.P.). R.C., M.C.N., S.M.B., S.K., W.F.F., I.M.K., J.M.G., R.H.S., L.L.G., C.S.R., Z.M., S.N., S.G., K.A.E., J.J.W., and H.S.P. are employees of Genentech, Inc., a member of the Roche group. This work was supported in part by a grant from Genentech to C.D.J., S.R.V., J.F.C., S.M., M.P., and C.C. M.P. has received research grant support from Genentech for clinical trials that were not related in any way with the research described in this manuscript.
Keywords
- CELLCYCLE
ASJC Scopus subject areas
- Oncology
- Cancer Research