A high-throughput induction gene trap approach defines C4ST as a target of BMP signaling

Michael Klüppel*, Katherine A. Vallis, Jeffrey L. Wrana

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Here we describe a novel gene trap protocol to screen for target genes that are regulated during inductive events in undifferentiated and differentiated mouse embryonic stem cells. This approach integrates several features that allows in vitro screening of large numbers of gene trap clones prior to generating lines of mutant mice. Moreover, targets of spatially and temporally restricted signaling pathways can be analyzed by screening undifferentiated ES cells versus ES cells differentiated into embryoid bodies. We employed this protocol to screen 1920 gene trap lines to identify targets and mediators of signaling through three growth factors of the TGFβ superfamily - BMP2, activin and nodal. We identified two genes that are induced by BMP2 in a differentiation-dependent manner. One of the genes encodes for Chondroitin-4-sulfotransferase and displays a highly specific temporal and spatial expression pattern during mouse embryogenesis. These results demonstrate the feasibility of a high-throughput gene trap approach as a means to identify mediators and targets of multiple growth factor signaling pathways that function during different stages of development.

Original languageEnglish (US)
Pages (from-to)77-89
Number of pages13
JournalMechanisms of Development
Volume118
Issue number1-2
DOIs
StatePublished - Oct 2002

Keywords

  • Bone morphogenetic protein
  • Chondroitin-4-sulfotransferase
  • Embryogenesis
  • Embryoid bodies
  • Embryonic stem cells
  • Expression pattern
  • Gene trapping
  • Target genes
  • Transforming growth factor β

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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