A high throughput platform for eukaryotic genes.

Yunjia Chen*, Shihong Qiu, Chi Hao Luan, Ming Luo

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The objective of structural proteomics is to determine the structures of all protein folds found in nature and develop a public resource to organize and analyze protein structures and fold families. High throughput (HTP) methods, which can process multiple samples in parallel, saving both time and cost, play important roles in achieving this goal. Using C. elegans and human as model organisms, a HTP cloning and expression pipeline was developed for structural proteomics that required production of a large number of recombinant proteins, applying the Gateway cloning/expression technology and utilizing a stepwise automation strategy on an integrated robotic platform. This system can process up to 384 unique samples in parallel and successfully automates most aspects of gene cloning and protein expression analysis, from PCR to protein solubility profiling.

Original languageEnglish (US)
Pages (from-to)209-220
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume426
DOIs
StatePublished - 2008

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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