A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile

Center for Structural Biology of Infectious Diseases

doi:10.1128/MRA.00507-23

A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile

Center for Structural Biology of Infectious Diseases

Research output: Contribution to journal › Article › peer-review

Abstract

Clostridioides difficile causes life-threatening gastrointestinal infections. It is a high-risk pathogen due to a lack of effective treatments, antimicrobial resistance, and a poorly conserved genomic core. Herein, we report 30 X-ray structures from a structure genomics pipeline spanning 13 years, representing 10.2% of the X-ray structures for this important pathogen.

Original language	English (US)
Journal	Microbiology Resource Announcements
Volume	12
Issue number	10
DOIs	https://doi.org/10.1128/MRA.00507-23
State	Published - Oct 2023

Funding

This project has been funded in whole or in part with federal funds from the Department of Health and Human Services, NIH, and National Institute of Allergy and Infectious Diseases under contract nos. HHSN272200700058C, HHSN272201200026C (to W.F.A.), and HHSN272201700060C and 75N93022C00035 (to K.J.F.S.). The Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory, and the Structural Biology Center beamline resources are supported under contract no. DE-AC02-06CH11357. Use of the LS-CAT Sector 21 was supported by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor (grant 085P1000817). The funders had no role in the conduct of research, data analysis, or manuscript preparation.

Keywords

Clostridiodes
Clostridium difficile
X-ray
pipeline
protein structure-function
structural genomics

ASJC Scopus subject areas

Immunology and Microbiology (miscellaneous)
Genetics
Molecular Biology

Access to Document

10.1128/MRA.00507-23

Cite this

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title = "A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile",

abstract = "Clostridioides difficile causes life-threatening gastrointestinal infections. It is a high-risk pathogen due to a lack of effective treatments, antimicrobial resistance, and a poorly conserved genomic core. Herein, we report 30 X-ray structures from a structure genomics pipeline spanning 13 years, representing 10.2% of the X-ray structures for this important pathogen.",

keywords = "Clostridiodes, Clostridium difficile, X-ray, pipeline, protein structure-function, structural genomics",

author = "{Center for Structural Biology of Infectious Diseases} and Monica Rosas-Lemus and Supratim Dey and George Minasov and Kemin Tan and Anderson, {Spencer M} and Brunzelle, {Joseph S} and Salvatore Nocadello and Ivan Shabalin and Filippova, {Ekaterina V} and Andrei Halavaty and Youngchang Kim and Natalia Maltseva and Jerzy Osipiuk and Wladek Minor and Andrzej Joachimiak and Alexei Savchenko and Anderson, {Wayne F} and Satchell, {Karla J.F.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2023 Rosas-Lemus et al.",

year = "2023",

month = oct,

doi = "10.1128/MRA.00507-23",

language = "English (US)",

volume = "12",

journal = "Microbiology Resource Announcements",

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AU - Center for Structural Biology of Infectious Diseases

AU - Rosas-Lemus, Monica

AU - Dey, Supratim

AU - Minasov, George

AU - Tan, Kemin

AU - Anderson, Spencer M

AU - Brunzelle, Joseph S

AU - Nocadello, Salvatore

AU - Shabalin, Ivan

AU - Filippova, Ekaterina V

AU - Halavaty, Andrei

AU - Kim, Youngchang

AU - Maltseva, Natalia

AU - Osipiuk, Jerzy

AU - Minor, Wladek

AU - Joachimiak, Andrzej

AU - Savchenko, Alexei

AU - Anderson, Wayne F

AU - Satchell, Karla J.F.

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AB - Clostridioides difficile causes life-threatening gastrointestinal infections. It is a high-risk pathogen due to a lack of effective treatments, antimicrobial resistance, and a poorly conserved genomic core. Herein, we report 30 X-ray structures from a structure genomics pipeline spanning 13 years, representing 10.2% of the X-ray structures for this important pathogen.

KW - Clostridiodes

KW - Clostridium difficile

KW - X-ray

KW - pipeline

KW - protein structure-function

KW - structural genomics

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A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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