A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli

Benjamin J. Des Soye; Vincent R. Gerbasi; Paul M. Thomas; Neil L. Kelleher; Michael C. Jewett

doi:10.1016/j.chembiol.2019.10.008

A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli

Benjamin J. Des Soye, Vincent R. Gerbasi, Paul M. Thomas, Neil L. Kelleher, Michael C. Jewett^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

3 Scopus citations

Abstract

Des Soye et al. create and optimize a strain of Escherichia coli that expresses T7 RNA polymerase so that lysates prepared from the strain are enriched with sufficient polymerase to catalyze high-yielding cell-free transcription and translation reactions. Using the resulting platform, the authors synthesize products containing up to 40 non-canonical amino acids.

Original language	English (US)
Pages (from-to)	1743-1754.e9
Journal	Cell Chemical Biology
Volume	26
Issue number	12
DOIs	https://doi.org/10.1016/j.chembiol.2019.10.008
State	Published - Dec 19 2019

Keywords

cell-free protein synthesis
chemical biology
genetic code expansion
genome engineering
in vitro transcription and translation
non-canonical amino acids
synthetic biology

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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Cite this

Des Soye, B. J., Gerbasi, V. R., Thomas, P. M., Kelleher, N. L., & Jewett, M. C. (2019). A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli. Cell Chemical Biology, 26(12), 1743-1754.e9. https://doi.org/10.1016/j.chembiol.2019.10.008

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