A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli

Benjamin J. Des Soye; Vincent R. Gerbasi; Paul M. Thomas; Neil L. Kelleher; Michael C. Jewett

doi:10.1016/j.chembiol.2019.10.008

A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli

Benjamin J. Des Soye, Vincent R. Gerbasi, Paul M. Thomas, Neil L. Kelleher, Michael C. Jewett^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

Abstract

Des Soye et al. create and optimize a strain of Escherichia coli that expresses T7 RNA polymerase so that lysates prepared from the strain are enriched with sufficient polymerase to catalyze high-yielding cell-free transcription and translation reactions. Using the resulting platform, the authors synthesize products containing up to 40 non-canonical amino acids.

Original language	English (US)
Pages (from-to)	1743-1754.e9
Journal	Cell Chemical Biology
Volume	26
Issue number	12
DOIs	https://doi.org/10.1016/j.chembiol.2019.10.008
State	Published - Dec 19 2019

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Keywords

cell-free protein synthesis
chemical biology
genetic code expansion
genome engineering
in vitro transcription and translation
non-canonical amino acids
synthetic biology

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Cite this

Des Soye, B. J., Gerbasi, V. R., Thomas, P. M., Kelleher, N. L., & Jewett, M. C. (2019). A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli. Cell Chemical Biology, 26(12), 1743-1754.e9. https://doi.org/10.1016/j.chembiol.2019.10.008

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Access to Document

10.1016/j.chembiol.2019.10.008