A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli

Benjamin J. Des Soye, Vincent R. Gerbasi, Paul M. Thomas, Neil L. Kelleher, Michael C. Jewett*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Des Soye et al. create and optimize a strain of Escherichia coli that expresses T7 RNA polymerase so that lysates prepared from the strain are enriched with sufficient polymerase to catalyze high-yielding cell-free transcription and translation reactions. Using the resulting platform, the authors synthesize products containing up to 40 non-canonical amino acids.

Original languageEnglish (US)
Pages (from-to)1743-1754.e9
JournalCell Chemical Biology
Volume26
Issue number12
DOIs
StatePublished - Dec 19 2019

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Keywords

  • cell-free protein synthesis
  • chemical biology
  • genetic code expansion
  • genome engineering
  • in vitro transcription and translation
  • non-canonical amino acids
  • synthetic biology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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