A homeobox gene of the Antennapedia class is required for human adult erythropoiesis

Kenichi Takeshita*, Jacques A. Bollekens, Nobuko Hijiya, Mariusz Ratajczak, Frank H. Ruddle, Alan M. Gewirtz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

In this report we investigate the role of homeobox genes of the Antennapedia class in adult erythropoiesis, the process by which erythrocytes are formed in the bone marrow. We initially identified Hox genes expressed in mouse erythroleukemia cells by a PCR technique using degenerate primers capable of detecting most of the known genes in Hox clusters 1-4. Four different transcripts, Hox-3.3, -3.5, -3.6, and -4.3 were identified. An antisense oligonucleotide directed against HOX3C (human homologue of Hox-3.3) was used to study the effect of the colony formation by human erythroid progenitor cells. The oligonucleotide inhibited the formation of colony-forming unit erythroid-derived colonies but did not affect the size or degree of hemoglobinization. The more primitive erythroid burst-forming unit colonies or myeloid colonies were not affected. These results show that Hox-3.3 is involved in an early step in the proliferation of the erythroid colony-forming unit subset of progenitor cells.

Original languageEnglish (US)
Pages (from-to)3535-3538
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number8
DOIs
StatePublished - Apr 15 1993

Keywords

  • Antisense
  • Erythroid colony-forming units
  • Hematopoiesis
  • Hox
  • Progenitor

ASJC Scopus subject areas

  • General

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