@article{58631ef2a97d4a8191634abd116e15dd,
title = "A human pluripotent stem cell surface N-glycoproteome resource reveals markers, extracellular epitopes, and drug targets",
abstract = "Detailed knowledge of cell-surface proteins for isolating well-defined populations of human pluripotent stem cells (hPSCs) would significantly enhance their characterization and translational potential. Through a chemoproteomic approach, we developed a cell-surface proteome inventory containing 496 N-linked glycoproteins on human embryonic (hESCs) and induced PSCs (hiPSCs). Against a backdrop of human fibroblasts and 50 other cell types, >100 surface proteins of interest for hPSCs were revealed. The >30 positive and negative markers verified here by orthogonal approaches provide experimental justification for the rational selection of pluripotency and lineage markers, epitopes for cell isolation, and reagents for the characterization of putative hiPSC lines. Comparative differences between the chemoproteomic-defined surfaceome and the transcriptome-predicted surfaceome directly led to the discovery that STF-31, a reported GLUT-1 inhibitor, is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures.",
author = "Boheler, {Kenneth R.} and Subarna Bhattacharya and Kropp, {Erin M.} and Sandra Chuppa and Riordon, {Daniel R.} and Damaris Bausch-Fluck and Burridge, {Paul W.} and Wu, {Joseph C.} and Wersto, {Robert P.} and Chan, {Godfrey Chi Fung} and Sridhar Rao and Bernd Wollscheid and Gundry, {Rebekah L.}",
note = "Funding Information: This research was supported by NIH 4R00HL094708-03, BD Biosciences Research Grant Award, MCW Research Affairs Committee New Faculty Award, and the Kern foundation (startup funds) at the Medical College of Wisconsin (R.L.G.); the Intramural Research Program of the NIH/NIA, NIH Induced Pluripotent Stem Cell Center/Center for Regenerative Medicine Research Study Award and Research Grants Council of Hong Kong Theme-based Research Scheme T13-706/11 (K.R.B.); the Swiss National Science Foundation (grants 31003A_135805 to B.W.); Institutional Research Grant #86-004 from the American Cancer Society and the Midwest Athletes against Childhood Cancer (S.R.); U01 HL099776 and AHA Established Investigator Award (J.C.W.); AHA Postdoctoral Fellowship 12POST12050254 (P.W.B.); E.M.K. is a member of the MCW-MSTP, which is partially supported by a T32 grant from NIGMS, GM080202. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank Hope Campbell at the Flow Cytometry Core of the Blood Research Institute of Wisconsin and Dr. Kate Noon, Michael Pereckas, and Xioagang Wu at the MCW Mass Spectrometry Facility for assistance with data collection. Special thanks to Dr. John Corbett (MCW) for generously providing access to the confocal microscope and the Biotechnology & Bioengineering Center (MCW) for access to the Real-Time PCR System. ",
year = "2014",
month = jul,
day = "8",
doi = "10.1016/j.stemcr.2014.05.002",
language = "English (US)",
volume = "3",
pages = "185--203",
journal = "Stem cell reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "1",
}