A human pluripotent stem cell surface N-glycoproteome resource reveals markers, extracellular epitopes, and drug targets

Kenneth R. Boheler*, Subarna Bhattacharya, Erin M. Kropp, Sandra Chuppa, Daniel R. Riordon, Damaris Bausch-Fluck, Paul W. Burridge, Joseph C. Wu, Robert P. Wersto, Godfrey Chi Fung Chan, Sridhar Rao, Bernd Wollscheid, Rebekah L. Gundry

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Detailed knowledge of cell-surface proteins for isolating well-defined populations of human pluripotent stem cells (hPSCs) would significantly enhance their characterization and translational potential. Through a chemoproteomic approach, we developed a cell-surface proteome inventory containing 496 N-linked glycoproteins on human embryonic (hESCs) and induced PSCs (hiPSCs). Against a backdrop of human fibroblasts and 50 other cell types, >100 surface proteins of interest for hPSCs were revealed. The >30 positive and negative markers verified here by orthogonal approaches provide experimental justification for the rational selection of pluripotency and lineage markers, epitopes for cell isolation, and reagents for the characterization of putative hiPSC lines. Comparative differences between the chemoproteomic-defined surfaceome and the transcriptome-predicted surfaceome directly led to the discovery that STF-31, a reported GLUT-1 inhibitor, is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures.

Original languageEnglish (US)
Pages (from-to)185-203
Number of pages19
JournalStem cell reports
Volume3
Issue number1
DOIs
StatePublished - Jul 8 2014

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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