TY - GEN
T1 - A Hydrogel Microneedle-Based Biosensor Integrating Aptamer Probes And Fluorescence Detection for Biomarker Quantification
AU - Zheng, Hanjia
AU - GhavamiNejad, Amin
AU - Poudineh, Mahla
N1 - Funding Information:
This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant and center for bioengineering and biotechnology (CBB) seed funding at the University of Waterloo.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - In this study, a hydrogel microneedle (HMN) biosensor functionalized with fluorescence labeled aptamer probes is developed for detecting biomarkers in skin interstitial fluid (ISF). A novel and rapid assay for attaching the single stranded aptamer probes to hydrogel matrix is integrated. Our HMN biosensor enables reagent-less and on-needle target detection with high sensitivity and specificity. Here we demonstrate the applications of this new biosensor for ex vitro adenosine triphosphate (ATP) and glucose detection using a skin-mimicking phantom gel.
AB - In this study, a hydrogel microneedle (HMN) biosensor functionalized with fluorescence labeled aptamer probes is developed for detecting biomarkers in skin interstitial fluid (ISF). A novel and rapid assay for attaching the single stranded aptamer probes to hydrogel matrix is integrated. Our HMN biosensor enables reagent-less and on-needle target detection with high sensitivity and specificity. Here we demonstrate the applications of this new biosensor for ex vitro adenosine triphosphate (ATP) and glucose detection using a skin-mimicking phantom gel.
KW - aptamer probe
KW - methacrylated hyaluronic acid (MeHA)
KW - microneedle
KW - transdermal biosensing
UR - http://www.scopus.com/inward/record.url?scp=85136922603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136922603&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136922603
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1433
EP - 1434
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -