A hypothalamic activator of calmodulin-dependent enzymes is thymosin β4 (1-39)

A. A. Galoyan; B. Ya Gurvits; L. A. Shuvalova; Michael T. Davis; John E. Shively; Terry D. Lee

doi:10.1007/BF00969011

A hypothalamic activator of calmodulin-dependent enzymes is thymosin β₄ (1-39)

A. A. Galoyan, B. Ya Gurvits, L. A. Shuvalova, Michael T. Davis, John E. Shively, Terry D. Lee^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A new class of stimulators of basal activity of a number of calmodulin-dependent enzymes have been previously isolated from bovine hypothalamus. One of these stimulators, denoted as C₃, has been purified to homogeneity by reverse phase HPLC and tentatively identified as thymosin β₄ (1-39) by mass spectrometry and Edman microsequence analysis. The stimulating effect of C₃ on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin α₁ and thymosin β₄ (16-38). Evidence is presented that all the indicated compounds are Ca²⁺-independent high-affinity MLCK stimulators. The potency of the stimulators in activating the enzyme was: C₃>β₄>(CaM+Ca²⁺>α₁.

Original language	English (US)
Pages (from-to)	773-777
Number of pages	5
Journal	Neurochemical Research
Volume	17
Issue number	8
DOIs	https://doi.org/10.1007/BF00969011
State	Published - Aug 1992

Keywords

Thymosin
calcium-calmodulin
myosin light chain kinase
phosphodiesterase

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

Access to Document

10.1007/BF00969011

Cite this

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title = "A hypothalamic activator of calmodulin-dependent enzymes is thymosin β4 (1-39)",

abstract = "A new class of stimulators of basal activity of a number of calmodulin-dependent enzymes have been previously isolated from bovine hypothalamus. One of these stimulators, denoted as C3, has been purified to homogeneity by reverse phase HPLC and tentatively identified as thymosin β4 (1-39) by mass spectrometry and Edman microsequence analysis. The stimulating effect of C3 on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin α1 and thymosin β4 (16-38). Evidence is presented that all the indicated compounds are Ca2+-independent high-affinity MLCK stimulators. The potency of the stimulators in activating the enzyme was: C3>β4>(CaM+Ca2+>α1.",

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T1 - A hypothalamic activator of calmodulin-dependent enzymes is thymosin β4 (1-39)

AU - Galoyan, A. A.

AU - Gurvits, B. Ya

AU - Shuvalova, L. A.

AU - Davis, Michael T.

AU - Shively, John E.

AU - Lee, Terry D.

PY - 1992/8

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AB - A new class of stimulators of basal activity of a number of calmodulin-dependent enzymes have been previously isolated from bovine hypothalamus. One of these stimulators, denoted as C3, has been purified to homogeneity by reverse phase HPLC and tentatively identified as thymosin β4 (1-39) by mass spectrometry and Edman microsequence analysis. The stimulating effect of C3 on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin α1 and thymosin β4 (16-38). Evidence is presented that all the indicated compounds are Ca2+-independent high-affinity MLCK stimulators. The potency of the stimulators in activating the enzyme was: C3>β4>(CaM+Ca2+>α1.

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KW - calcium-calmodulin

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KW - phosphodiesterase

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