A knowledge-based experimental design system for nucleic acid engineering

K. Jiang; J. Z. Heng; S. B. Higgins; D. M. Watterson; T. A. Craig; T. J. Lukas; L. J Van Eldik

doi:10.1093/bioinformatics/6.3.205

A knowledge-based experimental design system for nucleic acid engineering

K. Jiang^*, J. Z. Heng, S. B. Higgins, D. M. Watterson, T. A. Craig, T. J. Lukas, L. J Van Eldik

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Presented in this paper is a knowledge-based experimental design system that incorporates the domain expertise used in nucleic acid engineering, thus automating the processing of error-prone, laborious low-level work, and many decision-making steps, and guiding the biologist toward a workable plan. This allows the biologist to work at a higher abstraction level, concentrating on more fundamental, difficult and challenging problems directly related to protein structure-function relationships. Cassette-based site-directed mutagenesis and synthetic gene designs are used as examples to illustrate the utility of the knowledge-based system approach to experimental design.

Original language	English (US)
Pages (from-to)	205-212
Number of pages	8
Journal	Bioinformatics
Volume	6
Issue number	3
DOIs	https://doi.org/10.1093/bioinformatics/6.3.205
State	Published - Jul 1990

Funding

The authors wish to thank Drs Jacques Haiech and Daniel M Roberts for their suggestions and comments at the early stage of this development, Mr Michael Shoemaker for his useful suggestions, and Dr Thomas R.Harris for providing a rich research environment. This research was supported in part by NIH grant GM 30861 (to D.M.W.) and by the Harry H.Straus-Martha Washington Straus Foundation and the Barbara Ingalls Shoak Foundation.

ASJC Scopus subject areas

Statistics and Probability
Biochemistry
Molecular Biology
Computer Science Applications
Computational Theory and Mathematics
Computational Mathematics

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10.1093/bioinformatics/6.3.205

Cite this

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title = "A knowledge-based experimental design system for nucleic acid engineering",

abstract = "Presented in this paper is a knowledge-based experimental design system that incorporates the domain expertise used in nucleic acid engineering, thus automating the processing of error-prone, laborious low-level work, and many decision-making steps, and guiding the biologist toward a workable plan. This allows the biologist to work at a higher abstraction level, concentrating on more fundamental, difficult and challenging problems directly related to protein structure-function relationships. Cassette-based site-directed mutagenesis and synthetic gene designs are used as examples to illustrate the utility of the knowledge-based system approach to experimental design.",

author = "K. Jiang and Heng, {J. Z.} and Higgins, {S. B.} and Watterson, {D. M.} and Craig, {T. A.} and Lukas, {T. J.} and Eldik, {L. J Van}",

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AU - Higgins, S. B.

AU - Watterson, D. M.

AU - Craig, T. A.

AU - Lukas, T. J.

AU - Eldik, L. J Van

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N2 - Presented in this paper is a knowledge-based experimental design system that incorporates the domain expertise used in nucleic acid engineering, thus automating the processing of error-prone, laborious low-level work, and many decision-making steps, and guiding the biologist toward a workable plan. This allows the biologist to work at a higher abstraction level, concentrating on more fundamental, difficult and challenging problems directly related to protein structure-function relationships. Cassette-based site-directed mutagenesis and synthetic gene designs are used as examples to illustrate the utility of the knowledge-based system approach to experimental design.

AB - Presented in this paper is a knowledge-based experimental design system that incorporates the domain expertise used in nucleic acid engineering, thus automating the processing of error-prone, laborious low-level work, and many decision-making steps, and guiding the biologist toward a workable plan. This allows the biologist to work at a higher abstraction level, concentrating on more fundamental, difficult and challenging problems directly related to protein structure-function relationships. Cassette-based site-directed mutagenesis and synthetic gene designs are used as examples to illustrate the utility of the knowledge-based system approach to experimental design.

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A knowledge-based experimental design system for nucleic acid engineering

Abstract

Funding

ASJC Scopus subject areas

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