A lateral flow-based ultra-sensitive p24 HIV assay utilizing fluorescent microparticles

Arman Nabatiyan; Michael A. Baumann; Zaheer Parpia; David Kelso

doi:10.1097/QAI.0b013e3181c4b9d5

A lateral flow-based ultra-sensitive p24 HIV assay utilizing fluorescent microparticles

Arman Nabatiyan^*, Michael A. Baumann, Zaheer Parpia, David Kelso

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The current demand for HIV diagnostic tests in resource-limited settings is not being fully addressed by the currently available nucleic acid test or enzyme-linked immunosorbent assay-based p24 antigen assays. This is primarily due to their high cost, their requirement for controlled laboratory conditions and/or personnel, or the relatively long turn-around time for test results. Self performing lateral flow assays address all of these issues but to date have not reached the level of analytical sensitivity for HIV p24 demonstrated by current generation enzyme-linked immunosorbent assay technology. This report presents an initial prototype lateral flow assay for HIV p24 antigen capable of achieving subpicogram per milliliter limits of analytical sensitivity and requiring less than 40 minutes to yield results.

Original language	English (US)
Pages (from-to)	55-61
Number of pages	7
Journal	Journal of Acquired Immune Deficiency Syndromes
Volume	53
Issue number	1
DOIs	https://doi.org/10.1097/QAI.0b013e3181c4b9d5
State	Published - Jan 2010

Keywords

Acid-shock immune disruption
Fluorescent microparticles
HIV p24 assay
Lateral flow diagnostic

ASJC Scopus subject areas

Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1097/QAI.0b013e3181c4b9d5

Cite this

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abstract = "The current demand for HIV diagnostic tests in resource-limited settings is not being fully addressed by the currently available nucleic acid test or enzyme-linked immunosorbent assay-based p24 antigen assays. This is primarily due to their high cost, their requirement for controlled laboratory conditions and/or personnel, or the relatively long turn-around time for test results. Self performing lateral flow assays address all of these issues but to date have not reached the level of analytical sensitivity for HIV p24 demonstrated by current generation enzyme-linked immunosorbent assay technology. This report presents an initial prototype lateral flow assay for HIV p24 antigen capable of achieving subpicogram per milliliter limits of analytical sensitivity and requiring less than 40 minutes to yield results.",

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AB - The current demand for HIV diagnostic tests in resource-limited settings is not being fully addressed by the currently available nucleic acid test or enzyme-linked immunosorbent assay-based p24 antigen assays. This is primarily due to their high cost, their requirement for controlled laboratory conditions and/or personnel, or the relatively long turn-around time for test results. Self performing lateral flow assays address all of these issues but to date have not reached the level of analytical sensitivity for HIV p24 demonstrated by current generation enzyme-linked immunosorbent assay technology. This report presents an initial prototype lateral flow assay for HIV p24 antigen capable of achieving subpicogram per milliliter limits of analytical sensitivity and requiring less than 40 minutes to yield results.

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A lateral flow-based ultra-sensitive p24 HIV assay utilizing fluorescent microparticles

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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