A lymphatic defect causes ocular hypertension and glaucoma in mice

Benjamin R. Thomson, Stefan Heinen, Marie Jeansson, Asish K. Ghosh, Anees Fatima, Hoon Ki Sung, Tuncer Onay, Hui Chen, Shinji Yamaguchi, Aris N. Economides, Ann Flenniken, Nicholas W. Gale, Young Kwon Hong, Amani Fawzi, Xiaorong Liu, Tsutomu Kume, Susan E. Quaggin*

*Corresponding author for this work

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Glaucoma is a leading cause of blindness, afflicting more than 60 million people worldwide. Increased intraocular pressure (IOP) due to impaired aqueous humor drainage is a major risk factor for the development of glaucoma. Here, we demonstrated that genetic disruption of the angiopoietin/TIE2 (ANGPT/TIE2) signaling pathway results in high IOP, buphthalmos, and classic features of glaucoma, including retinal ganglion degeneration and vision loss. Eyes from mice with induced deletion of Angpt1 and Angpt2 (A1A2FloxWB mice) lacked drainage pathways in the corneal limbus, including Schlemm's canal and lymphatic capillaries, which share expression of the PROX1, VEGFR3, and FOXC family of transcription factors. VEGFR3 and FOXCs have been linked to lymphatic disorders in patients, and FOXC1 has been linked to glaucoma. In contrast to blood endothelium, in which ANGPT2 is an antagonist of ANGPT1, we have shown that both ligands cooperate to regulate TIE2 in the lymphatic network of the eye. While A1A2FloxWB mice developed high IOP and glaucoma, expression of ANGPT1 or ANGPT2 alone was sufficient for ocular drainage. Furthermore, we demonstrated that loss of FOXC2 from lymphatics results in TIE2 downregulation, suggesting a mechanism for ocular defects in patients with FOXC mutations. These data reveal a pathogenetic and molecular basis for glaucoma and demonstrate the importance of angiopoietin ligand cooperation in the lymphatic endothelium.

Original languageEnglish (US)
Pages (from-to)4320-4324
Number of pages5
JournalJournal of Clinical Investigation
Volume124
Issue number10
DOIs
StatePublished - Oct 1 2014

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Ocular Hypertension
Glaucoma
Intraocular Pressure
Angiopoietins
Drainage
Hydrophthalmos
Lymphatic Endothelium
Limbus Corneae
Ligands
Retinal Degeneration
Lymphatic Vessels
Aqueous Humor
Blindness
Ganglia
Endothelium
Transcription Factors
Down-Regulation
Mutation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Thomson, Benjamin R. ; Heinen, Stefan ; Jeansson, Marie ; Ghosh, Asish K. ; Fatima, Anees ; Sung, Hoon Ki ; Onay, Tuncer ; Chen, Hui ; Yamaguchi, Shinji ; Economides, Aris N. ; Flenniken, Ann ; Gale, Nicholas W. ; Hong, Young Kwon ; Fawzi, Amani ; Liu, Xiaorong ; Kume, Tsutomu ; Quaggin, Susan E. / A lymphatic defect causes ocular hypertension and glaucoma in mice. In: Journal of Clinical Investigation. 2014 ; Vol. 124, No. 10. pp. 4320-4324.
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abstract = "Glaucoma is a leading cause of blindness, afflicting more than 60 million people worldwide. Increased intraocular pressure (IOP) due to impaired aqueous humor drainage is a major risk factor for the development of glaucoma. Here, we demonstrated that genetic disruption of the angiopoietin/TIE2 (ANGPT/TIE2) signaling pathway results in high IOP, buphthalmos, and classic features of glaucoma, including retinal ganglion degeneration and vision loss. Eyes from mice with induced deletion of Angpt1 and Angpt2 (A1A2FloxWB mice) lacked drainage pathways in the corneal limbus, including Schlemm's canal and lymphatic capillaries, which share expression of the PROX1, VEGFR3, and FOXC family of transcription factors. VEGFR3 and FOXCs have been linked to lymphatic disorders in patients, and FOXC1 has been linked to glaucoma. In contrast to blood endothelium, in which ANGPT2 is an antagonist of ANGPT1, we have shown that both ligands cooperate to regulate TIE2 in the lymphatic network of the eye. While A1A2FloxWB mice developed high IOP and glaucoma, expression of ANGPT1 or ANGPT2 alone was sufficient for ocular drainage. Furthermore, we demonstrated that loss of FOXC2 from lymphatics results in TIE2 downregulation, suggesting a mechanism for ocular defects in patients with FOXC mutations. These data reveal a pathogenetic and molecular basis for glaucoma and demonstrate the importance of angiopoietin ligand cooperation in the lymphatic endothelium.",
author = "Thomson, {Benjamin R.} and Stefan Heinen and Marie Jeansson and Ghosh, {Asish K.} and Anees Fatima and Sung, {Hoon Ki} and Tuncer Onay and Hui Chen and Shinji Yamaguchi and Economides, {Aris N.} and Ann Flenniken and Gale, {Nicholas W.} and Hong, {Young Kwon} and Amani Fawzi and Xiaorong Liu and Tsutomu Kume and Quaggin, {Susan E.}",
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Thomson, BR, Heinen, S, Jeansson, M, Ghosh, AK, Fatima, A, Sung, HK, Onay, T, Chen, H, Yamaguchi, S, Economides, AN, Flenniken, A, Gale, NW, Hong, YK, Fawzi, A, Liu, X, Kume, T & Quaggin, SE 2014, 'A lymphatic defect causes ocular hypertension and glaucoma in mice', Journal of Clinical Investigation, vol. 124, no. 10, pp. 4320-4324. https://doi.org/10.1172/JCI77162

A lymphatic defect causes ocular hypertension and glaucoma in mice. / Thomson, Benjamin R.; Heinen, Stefan; Jeansson, Marie; Ghosh, Asish K.; Fatima, Anees; Sung, Hoon Ki; Onay, Tuncer; Chen, Hui; Yamaguchi, Shinji; Economides, Aris N.; Flenniken, Ann; Gale, Nicholas W.; Hong, Young Kwon; Fawzi, Amani; Liu, Xiaorong; Kume, Tsutomu; Quaggin, Susan E.

In: Journal of Clinical Investigation, Vol. 124, No. 10, 01.10.2014, p. 4320-4324.

Research output: Contribution to journalArticle

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T1 - A lymphatic defect causes ocular hypertension and glaucoma in mice

AU - Thomson, Benjamin R.

AU - Heinen, Stefan

AU - Jeansson, Marie

AU - Ghosh, Asish K.

AU - Fatima, Anees

AU - Sung, Hoon Ki

AU - Onay, Tuncer

AU - Chen, Hui

AU - Yamaguchi, Shinji

AU - Economides, Aris N.

AU - Flenniken, Ann

AU - Gale, Nicholas W.

AU - Hong, Young Kwon

AU - Fawzi, Amani

AU - Liu, Xiaorong

AU - Kume, Tsutomu

AU - Quaggin, Susan E.

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Glaucoma is a leading cause of blindness, afflicting more than 60 million people worldwide. Increased intraocular pressure (IOP) due to impaired aqueous humor drainage is a major risk factor for the development of glaucoma. Here, we demonstrated that genetic disruption of the angiopoietin/TIE2 (ANGPT/TIE2) signaling pathway results in high IOP, buphthalmos, and classic features of glaucoma, including retinal ganglion degeneration and vision loss. Eyes from mice with induced deletion of Angpt1 and Angpt2 (A1A2FloxWB mice) lacked drainage pathways in the corneal limbus, including Schlemm's canal and lymphatic capillaries, which share expression of the PROX1, VEGFR3, and FOXC family of transcription factors. VEGFR3 and FOXCs have been linked to lymphatic disorders in patients, and FOXC1 has been linked to glaucoma. In contrast to blood endothelium, in which ANGPT2 is an antagonist of ANGPT1, we have shown that both ligands cooperate to regulate TIE2 in the lymphatic network of the eye. While A1A2FloxWB mice developed high IOP and glaucoma, expression of ANGPT1 or ANGPT2 alone was sufficient for ocular drainage. Furthermore, we demonstrated that loss of FOXC2 from lymphatics results in TIE2 downregulation, suggesting a mechanism for ocular defects in patients with FOXC mutations. These data reveal a pathogenetic and molecular basis for glaucoma and demonstrate the importance of angiopoietin ligand cooperation in the lymphatic endothelium.

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