A map of nucleosome positions in yeast at base-pair resolution

Kristin Brogaard, Liqun Xi, Ji Ping Wang*, Jonathan Widom

*Corresponding author for this work

Research output: Contribution to journalArticle

236 Scopus citations

Abstract

The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.

Original languageEnglish (US)
Pages (from-to)496-501
Number of pages6
JournalNature
Volume486
Issue number7404
DOIs
StatePublished - Jun 28 2012

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ASJC Scopus subject areas

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