A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase

Xingliang Lu; Dejan Nikolic; Deanna J. Mitchell; Richard B. Van Breemen; John A. Mersfelder; Russ Hille; Richard B. Silverman

doi:10.1016/j.bmcl.2003.07.032

A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase

Xingliang Lu, Dejan Nikolic, Deanna J. Mitchell, Richard B. Van Breemen, John A. Mersfelder, Russ Hille, Richard B. Silverman^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H₂¹⁸O and ¹⁸O₂, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.

Original language	English (US)
Pages (from-to)	4129-4132
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	13
Issue number	22
DOIs	https://doi.org/10.1016/j.bmcl.2003.07.032
State	Published - Nov 17 2003

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2003.07.032

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title = "A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase",

abstract = "Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and 18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.",

author = "Xingliang Lu and Dejan Nikolic and Mitchell, {Deanna J.} and {Van Breemen}, {Richard B.} and Mersfelder, {John A.} and Russ Hille and Silverman, {Richard B.}",

note = "Funding Information: The authors are grateful to the National Institutes of Health for research grants to R.B.S. (GM32634) and to R.H. (GM58481) and an Instrument Grant to R.B.v.B (RR10485). ",

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doi = "10.1016/j.bmcl.2003.07.032",

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TY - JOUR

T1 - A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase

AU - Lu, Xingliang

AU - Nikolic, Dejan

AU - Mitchell, Deanna J.

AU - Van Breemen, Richard B.

AU - Mersfelder, John A.

AU - Hille, Russ

AU - Silverman, Richard B.

N1 - Funding Information: The authors are grateful to the National Institutes of Health for research grants to R.B.S. (GM32634) and to R.H. (GM58481) and an Instrument Grant to R.B.v.B (RR10485).

PY - 2003/11/17

Y1 - 2003/11/17

N2 - Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and 18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.

AB - Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and 18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.

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JF - Bioorganic and Medicinal Chemistry Letters

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ER -

A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase

Abstract

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