A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase

Xingliang Lu, Dejan Nikolic, Deanna J. Mitchell, Richard B. Van Breemen, John A. Mersfelder, Russ Hille, Richard B Silverman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and 18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.

Original languageEnglish (US)
Pages (from-to)4129-4132
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume13
Issue number22
DOIs
StatePublished - Nov 17 2003

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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