TY - JOUR
T1 - A mechanism for substrate-induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase
AU - Lu, Xingliang
AU - Nikolic, Dejan
AU - Mitchell, Deanna J.
AU - Van Breemen, Richard B.
AU - Mersfelder, John A.
AU - Hille, Russ
AU - Silverman, Richard B
PY - 2003/11/17
Y1 - 2003/11/17
N2 - Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and 18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.
AB - Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and 18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.
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U2 - 10.1016/j.bmcl.2003.07.032
DO - 10.1016/j.bmcl.2003.07.032
M3 - Article
C2 - 14592522
AN - SCOPUS:0242380212
VL - 13
SP - 4129
EP - 4132
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
SN - 0960-894X
IS - 22
ER -