A membrane logic gate to control secretion from vesicles

Claire H. Hilburger, Kamryn L. Lewis, Miranda L. Jacobs, Neha P. Kamat

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: There has been considerable interest in the design of synthetic vesicles that can both synthesize and deliver bioactive payloads for technological and therapeutic applications. Channel proteins, such as α-hemolysin (αHL), that integrate into vesicle membranes provide a strategy to release encapsulated molecules. In addition, channel proteins can be expressed via cell-free expression systems such that channel functions can be coupled to genetic circuits. It has been difficult, however, to control precisely when channel proteins integrate into vesicle membranes. Here, we present the design of a membrane AND gate in which the presence of both a membrane-associating lipid and a channel protein lead to the functional assembly of the channel protein into phospholipid vesicle membranes. Using dye release assays, we characterized the propensity for αHL to integrate into vesicles as a function of cholesterol and OA content, and αHL concentration. We explored how the addition of OA micelles to phospholipid vesicles enables changes in membrane composition that promote pore formation. Finally, we explored the compatibility of our membrane design with a cell-free expression system in which αHL is expressed.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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    Hilburger, C. H., Lewis, K. L., Jacobs, M. L., & Kamat, N. P. (2019). A membrane logic gate to control secretion from vesicles. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.