A method for characterizing adsorption of flowing solutes to microfluidic device surfaces

Kenneth R. Hawkins; Mark R. Steedman; Richard R. Baldwin; Elain Fu; Sandip Ghosal; Paul Yager

doi:10.1039/b612894g

A method for characterizing adsorption of flowing solutes to microfluidic device surfaces

Kenneth R. Hawkins^*, Mark R. Steedman, Richard R. Baldwin, Elain Fu, Sandip Ghosal, Paul Yager

^*Corresponding author for this work

Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH₃O(CH ₂CH₂O)₄CH₃), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise.

Original language	English (US)
Pages (from-to)	281-285
Number of pages	5
Journal	Lab on a Chip
Volume	7
Issue number	2
DOIs	https://doi.org/10.1039/b612894g
State	Published - 2007

ASJC Scopus subject areas

Bioengineering
Biochemistry
Chemistry(all)
Biomedical Engineering

Access to Document

10.1039/b612894g

Cite this

@article{96abfc65b6634ccb8136a940c1e5c6d3,

title = "A method for characterizing adsorption of flowing solutes to microfluidic device surfaces",

abstract = "We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH3O(CH 2CH2O)4CH3), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise.",

author = "Hawkins, {Kenneth R.} and Steedman, {Mark R.} and Baldwin, {Richard R.} and Elain Fu and Sandip Ghosal and Paul Yager",

year = "2007",

doi = "10.1039/b612894g",

language = "English (US)",

volume = "7",

pages = "281--285",

journal = "Lab on a Chip",

issn = "1473-0197",

publisher = "Royal Society of Chemistry",

number = "2",

}

TY - JOUR

T1 - A method for characterizing adsorption of flowing solutes to microfluidic device surfaces

AU - Hawkins, Kenneth R.

AU - Steedman, Mark R.

AU - Baldwin, Richard R.

AU - Fu, Elain

AU - Ghosal, Sandip

AU - Yager, Paul

PY - 2007

Y1 - 2007

N2 - We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH3O(CH 2CH2O)4CH3), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise.

AB - We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH3O(CH 2CH2O)4CH3), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise.

UR - http://www.scopus.com/inward/record.url?scp=33846894515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846894515&partnerID=8YFLogxK

U2 - 10.1039/b612894g

DO - 10.1039/b612894g

M3 - Article

C2 - 17268632

AN - SCOPUS:33846894515

SN - 1473-0197

VL - 7

SP - 281

EP - 285

JO - Lab on a Chip

JF - Lab on a Chip

IS - 2

ER -

A method for characterizing adsorption of flowing solutes to microfluidic device surfaces

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this