A method for characterizing adsorption of flowing solutes to microfluidic device surfaces

Kenneth R. Hawkins*, Mark R. Steedman, Richard R. Baldwin, Elain Fu, Sandip Ghosal, Paul Yager

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH3O(CH 2CH2O)4CH3), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise.

Original languageEnglish (US)
Pages (from-to)281-285
Number of pages5
JournalLab on a Chip
Volume7
Issue number2
DOIs
StatePublished - Jan 1 2007

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ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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