A Method for Dinitrochlorobenzene Contact Sensitization: A Clinicopathological Study

W. J. Catalona, P. T. Taylor, A. S. Rabson, P. B. Chretien

Research output: Contribution to journalArticle

158 Scopus citations


To facilitate measurement of cell-mediated immune responses, a method for quantitative clinical evaluation of contact sensitization to dinitrochlorobenzene (DNCB) was devised and used in 40 patients with cancer. When a sensitizing dose is applied, the immediate reaction produced expresses a general inflammatory response. Sensitization is expressed by the occurrence of a spontaneous flare seven to 14 days later, or by the reaction to a challenge dose of DNCB. Equivocal reactions require histologic examination. Use of a sensitizing dose of 2000 μg and a relatively weak challenge dose of 50 μg yielded a satisfactory incidence of sensitization with a low proportion of irritative inflammatory reactions.

Original languageEnglish (US)
Pages (from-to)399-402
Number of pages4
JournalNew England Journal of Medicine
Issue number8
StatePublished - Feb 24 1972

ASJC Scopus subject areas

  • Medicine(all)

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