@article{53fec21cc5e34b2697d73698745e4cb3,
title = "A method for large-scale, high-yield isolation of canine pancreatic islets of Langerhans",
abstract = "A modified collagenase digestion method is described for the isolation of large numbers of islets from the canine pancreas (∼3500 islets/g). The islets isolated by this technique remained viable and released insulin in response to secretagogues after one week of maintenance in tissue culture. Islets isolated from a single donor pancreas were re-implanted into the spleen of the same animal made diabetic by subtotal pancreatectomy and two injections of streptozotocin. Hyperglycemia was corrected in two dogs and decreased in a third dog followed for 30 days. The islet isolation method described, therefore, provides a sufficiently large yield of viable islets from one donor pancreas to correct or improve diabetes in a recipient animal.",
author = "Jack Noel and Alexander Rabinovitch and Les Olson and George Kyriakides and Joshua Miller and Mintz, {Daniel H.}",
note = "Funding Information: From the Division of Endocrinology, Department of Medicine and the Division of Transplantation, Department of Surgery, University of Miami School of Medicine and the Miami Veterans Administration Hospital. Miami. Florida. Received for publication May 4. 1981. Supported in part by Grants ROI-AM25243. ROI-AM25802. and Rot-AM25832 of NIAMDD. by an NIH research career development award 5-KO4-AM00552 (Dr. Rabinovitch), by the Diabetes Research Institute Foundation, and by the Veterans Administration Address reprint requests to Alex Rabinovitch. M.D., Department of Medicine (R-134). University of Miami School of Medicine, P.O. Box 016960. Miami, Florida 33101. o 1982 by Grune & Stratton, Inc. 0026-0495/82/3102-0014/$01.00/0",
year = "1982",
month = feb,
doi = "10.1016/0026-0495(82)90133-0",
language = "English (US)",
volume = "31",
pages = "184--187",
journal = "Metabolism",
issn = "0026-0495",
publisher = "W.B. Saunders",
number = "2",
}
