A method for large-scale, high-yield isolation of canine pancreatic islets of Langerhans

Jack Noel, Alexander Rabinovitch*, Les Olson, George Kyriakides, Joshua Miller, Daniel H. Mintz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

A modified collagenase digestion method is described for the isolation of large numbers of islets from the canine pancreas (∼3500 islets/g). The islets isolated by this technique remained viable and released insulin in response to secretagogues after one week of maintenance in tissue culture. Islets isolated from a single donor pancreas were re-implanted into the spleen of the same animal made diabetic by subtotal pancreatectomy and two injections of streptozotocin. Hyperglycemia was corrected in two dogs and decreased in a third dog followed for 30 days. The islet isolation method described, therefore, provides a sufficiently large yield of viable islets from one donor pancreas to correct or improve diabetes in a recipient animal.

Original languageEnglish (US)
Pages (from-to)184-187
Number of pages4
JournalMetabolism
Volume31
Issue number2
DOIs
StatePublished - Feb 1982

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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