A method for rapid generation of transgenic animals to evaluate testis genes during sexual maturation

Subeer S. Majumdar*, Abul Usmani, Indrashis Bhattacharya, Kanchan Sarda, Mukkesh Gautam, Deepika Sharma, Sayon Basu, Suveera Dhup

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


In certain forms of idiopathic infertility, there is failure of follicle stimulating hormone (FSH) and testosterone (T) to initiate spermatogenesis despite the presence of Sertoli cells and germ cells in the testis. In postnatal rats (up to 11 days of age) and infant monkeys (3-4 months old), robust division and differentiation of spermatogonial stem cells is not discerned, even though serum levels of FSH and T are similar to those found during adulthood. Lack of spermatogenesis together with normal hormone levels is a situation similar to that found in certain categories of male infertility. To investigate this intriguing situation, Sertoli cells were cultured from infant and pubertal rats and monkeys and differential gene expression by testicular Sertoli cells was evaluated by DNA microarray using the Agilent microarray system. To determine the role of candidate genes in regulation of spermatogenesis, transgenic animals over-expressing these genes must be generated. However, present techniques for generation of transgenic animals have limited utility for production of several transgenic animals within a short period of time. Therefore, we have developed a technique for making transgenic animals by the testicular route which is less labor intensive and less time consuming. This technique is also ethically superior since fewer mice are required than in existing alternative methods of transgenesis.

Original languageEnglish (US)
Pages (from-to)36-39
Number of pages4
JournalJournal of Reproductive Immunology
Issue number1-2
StatePublished - Jan 2009
Externally publishedYes


  • Gene expression
  • Sertoli cell
  • Sexual maturation
  • Transgenic animals

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology


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