A methodology for preparing nanostructured biomolecular interfaces with high enzymatic activity

Lu Shin Wong, Chinnan V. Karthikeyan, Daniel J. Eichelsdoerfer, Jason Micklefield, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The development of a novel method for functionalizing nanopatterned surfaces with catalytically active proteins is reported. This method involves using dip-pen nanolithography (DPN) and polymer pen lithography (PPL) to generate nanoscale patterns of coenzyme A, followed by a phosphopantetheinyl transferase-mediated coupling between coenzyme A and proteins fused to the ybbR-tag. By exploiting the ability to generate protein features over large areas afforded by DPN and PPL, it was now possible to measure protein activity directly on these surfaces. It was found that proteins immobilized on the nanoscale features not only display higher activity per area with decreasing feature size, but are also robust and can be used for repeated catalytic cycles. The immobilization method is applicable to a variety of proteins and gives rise to superior activity compared to proteins attached in random orientations on the surface.

Original languageEnglish (US)
Pages (from-to)659-666
Number of pages8
JournalNanoscale
Volume4
Issue number2
DOIs
StatePublished - Jan 21 2012

ASJC Scopus subject areas

  • Materials Science(all)

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