A microfluidic chip that sequentially processes microliter volumes to detect variable concentrations of protein analytes was described. Application of a hydrophilic coating to the separation area of the chip proved to be effective in the reduction of the adsorptive interactions of the target protein with the glass and polydimethylsiloxane (PDMS) surfaces. A linear relationship was observed between the signal intensity and protein concentration over the entire testing range. In the second generation chip, a design that allowed to test multiple samples in parallel was also developed.
|Number of pages
|Published - 2004
|2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004 → Nov 12 2004
|2004 AIChE Annual Meeting
|11/7/04 → 11/12/04
ASJC Scopus subject areas