A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle

Shuo Xiao; Jonathan R. Coppeta; Hunter B. Rogers; Brett C. Isenberg; Jie Zhu; Susan A. Olalekan; Kelly E. McKinnon; Danijela Dokic; Alexandra S. Rashedi; Daniel J. Haisenleder; Saurabh S. Malpani; Chanel A. Arnold-Murray; Kuanwei Chen; Mingyang Jiang; Lu Bai; Catherine T. Nguyen; Jiyang Zhang; Monica M. Laronda; Thomas J. Hope; Kruti P. Maniar; Mary Ellen Pavone; Michael J. Avram; Elizabeth C. Sefton; Spiro Getsios; Joanna E. Burdette; J. Julie Kim; Jeffrey T. Borenstein; Teresa K. Woodruff

doi:10.1038/ncomms14584

A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle

Shuo Xiao, Jonathan R. Coppeta, Hunter B. Rogers, Brett C. Isenberg, Jie Zhu, Susan A. Olalekan, Kelly E. McKinnon, Danijela Dokic, Alexandra S. Rashedi, Daniel J. Haisenleder, Saurabh S. Malpani, Chanel A. Arnold-Murray, Kuanwei Chen, Mingyang Jiang, Lu Bai, Catherine T. Nguyen, Jiyang Zhang, Monica M. Laronda, Thomas J. Hope, Kruti P. ManiarMary Ellen Pavone, Michael J. Avram, Elizabeth C. Sefton, Spiro Getsios, Joanna E. Burdette, J. Julie Kim, Jeffrey T. Borenstein, Teresa K. Woodruff^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

140 Scopus citations

Abstract

The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.

Original language	English (US)
Article number	14584
Journal	Nature communications
Volume	8
DOIs	https://doi.org/10.1038/ncomms14584
State	Published - Mar 28 2017

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

Access to Document

10.1038/ncomms14584

Fingerprint Dive into the research topics of 'A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle'. Together they form a unique fingerprint.

Cite this

Xiao, S., Coppeta, J. R., Rogers, H. B., Isenberg, B. C., Zhu, J., Olalekan, S. A., McKinnon, K. E., Dokic, D., Rashedi, A. S., Haisenleder, D. J., Malpani, S. S., Arnold-Murray, C. A., Chen, K., Jiang, M., Bai, L., Nguyen, C. T., Zhang, J., Laronda, M. M., Hope, T. J., ... Woodruff, T. K. (2017). A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle. Nature communications, 8, [14584]. https://doi.org/10.1038/ncomms14584

Xiao, Shuo ; Coppeta, Jonathan R. ; Rogers, Hunter B. ; Isenberg, Brett C. ; Zhu, Jie ; Olalekan, Susan A. ; McKinnon, Kelly E. ; Dokic, Danijela ; Rashedi, Alexandra S. ; Haisenleder, Daniel J. ; Malpani, Saurabh S. ; Arnold-Murray, Chanel A. ; Chen, Kuanwei ; Jiang, Mingyang ; Bai, Lu ; Nguyen, Catherine T. ; Zhang, Jiyang ; Laronda, Monica M. ; Hope, Thomas J. ; Maniar, Kruti P. ; Pavone, Mary Ellen ; Avram, Michael J. ; Sefton, Elizabeth C. ; Getsios, Spiro ; Burdette, Joanna E. ; Kim, J. Julie ; Borenstein, Jeffrey T. ; Woodruff, Teresa K. / A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle. In: Nature communications. 2017 ; Vol. 8.

@article{e10d5dc3261b4ad49f3eeb5e613c3c88,

title = "A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle",

abstract = "The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.",

author = "Shuo Xiao and Coppeta, {Jonathan R.} and Rogers, {Hunter B.} and Isenberg, {Brett C.} and Jie Zhu and Olalekan, {Susan A.} and McKinnon, {Kelly E.} and Danijela Dokic and Rashedi, {Alexandra S.} and Haisenleder, {Daniel J.} and Malpani, {Saurabh S.} and Arnold-Murray, {Chanel A.} and Kuanwei Chen and Mingyang Jiang and Lu Bai and Nguyen, {Catherine T.} and Jiyang Zhang and Laronda, {Monica M.} and Hope, {Thomas J.} and Maniar, {Kruti P.} and Pavone, {Mary Ellen} and Avram, {Michael J.} and Sefton, {Elizabeth C.} and Spiro Getsios and Burdette, {Joanna E.} and Kim, {J. Julie} and Borenstein, {Jeffrey T.} and Woodruff, {Teresa K.}",

note = "Funding Information: We thank all patients who donated their reproductive tissues through the Gynecological Tissue Library in Northwestern University, A.J. Spencer and J.Q. Santos for assistance with fabrication and testing of microfluidic devices, and R.N. Shah and A. Rutz kindly provided the 3D-printed scaffold for liver microtissue culture. This work was supported by NIEHS/ORWH/UH2ES022920; NCATS/NIEHS/NICHD/ORWH/UH3TR001207; and the NIH Common Fund",

year = "2017",

month = mar,

day = "28",

doi = "10.1038/ncomms14584",

language = "English (US)",

volume = "8",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

Xiao, S, Coppeta, JR, Rogers, HB, Isenberg, BC, Zhu, J, Olalekan, SA, McKinnon, KE, Dokic, D, Rashedi, AS, Haisenleder, DJ, Malpani, SS, Arnold-Murray, CA, Chen, K, Jiang, M, Bai, L, Nguyen, CT, Zhang, J, Laronda, MM , Hope, TJ , Maniar, KP , Pavone, ME , Avram, MJ, Sefton, EC, Getsios, S, Burdette, JE, Kim, JJ, Borenstein, JT & Woodruff, TK 2017, 'A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle', Nature communications, vol. 8, 14584. https://doi.org/10.1038/ncomms14584

A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle. / Xiao, Shuo; Coppeta, Jonathan R.; Rogers, Hunter B.; Isenberg, Brett C.; Zhu, Jie; Olalekan, Susan A.; McKinnon, Kelly E.; Dokic, Danijela; Rashedi, Alexandra S.; Haisenleder, Daniel J.; Malpani, Saurabh S.; Arnold-Murray, Chanel A.; Chen, Kuanwei; Jiang, Mingyang; Bai, Lu; Nguyen, Catherine T.; Zhang, Jiyang; Laronda, Monica M.; Hope, Thomas J.; Maniar, Kruti P.; Pavone, Mary Ellen ; Avram, Michael J.; Sefton, Elizabeth C.; Getsios, Spiro; Burdette, Joanna E.; Kim, J. Julie; Borenstein, Jeffrey T.; Woodruff, Teresa K.

In: Nature communications, Vol. 8, 14584, 28.03.2017.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle

AU - Xiao, Shuo

AU - Coppeta, Jonathan R.

AU - Rogers, Hunter B.

AU - Isenberg, Brett C.

AU - Zhu, Jie

AU - Olalekan, Susan A.

AU - McKinnon, Kelly E.

AU - Dokic, Danijela

AU - Rashedi, Alexandra S.

AU - Haisenleder, Daniel J.

AU - Malpani, Saurabh S.

AU - Arnold-Murray, Chanel A.

AU - Chen, Kuanwei

AU - Jiang, Mingyang

AU - Bai, Lu

AU - Nguyen, Catherine T.

AU - Zhang, Jiyang

AU - Laronda, Monica M.

AU - Hope, Thomas J.

AU - Maniar, Kruti P.

AU - Pavone, Mary Ellen

AU - Avram, Michael J.

AU - Sefton, Elizabeth C.

AU - Getsios, Spiro

AU - Burdette, Joanna E.

AU - Kim, J. Julie

AU - Borenstein, Jeffrey T.

AU - Woodruff, Teresa K.

N1 - Funding Information: We thank all patients who donated their reproductive tissues through the Gynecological Tissue Library in Northwestern University, A.J. Spencer and J.Q. Santos for assistance with fabrication and testing of microfluidic devices, and R.N. Shah and A. Rutz kindly provided the 3D-printed scaffold for liver microtissue culture. This work was supported by NIEHS/ORWH/UH2ES022920; NCATS/NIEHS/NICHD/ORWH/UH3TR001207; and the NIH Common Fund

PY - 2017/3/28

Y1 - 2017/3/28

N2 - The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.

AB - The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.

UR - http://www.scopus.com/inward/record.url?scp=85016461284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016461284&partnerID=8YFLogxK

U2 - 10.1038/ncomms14584

DO - 10.1038/ncomms14584

M3 - Article

C2 - 28350383

AN - SCOPUS:85016461284

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 14584

ER -