Abstract
The biobarcode assay (BCA) is capable of achieving low detection limits and high specificity for both protein and DNA targets. The realization of a BCA in a microfluidic format presents unique opportunities and challenges. In this work, we describe a modified form of the BCA called the surface immobilized biobarcode assay (SI-BCA). The SI-BCA employs microchannel walls functionalized with antibodies that bind with the intended targets. Compared with the conventional BCA, it reduces the system complexity and results in shortened process time, which is attributed to significantly reduced diffusion times in the micro-scale channels. Raw serum samples, without any pretreatment, were evaluated with this technique. Prostate specific antigen in the samples was detected at concentrations ranging from 40 pM to 40 fM. The detection limit of the assay using buffer samples is 10 fM. The entire assay, from sample injection to final data analysis was completed in 80 min.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2397-2403 |
| Number of pages | 7 |
| Journal | Biosensors and Bioelectronics |
| Volume | 24 |
| Issue number | 8 |
| DOIs | |
| State | Published - Apr 15 2009 |
Funding
This work was supported by the Nanoscale Science and Engineering Initiative of the National Science Foundation under NSF Award Number EEC-0118025 and by the Center of Cancer Nanotechnology Excellence initiative of the National Institutes of Health’s National Cancer Institute under Award Number U54CA119341. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect those of the National Science Foundation or the National Institutes of Health.
Keywords
- Biobarcode assay (BCA)
- Microfluidic
- Nanoparticle
- Prostate specific antigen (PSA)
- Serum
- Surface immobilized
ASJC Scopus subject areas
- Biophysics
- Biotechnology
- Biomedical Engineering
- Electrochemistry