TY - JOUR
T1 - A microRNA panel that regulates proinflammatory cytokines as diagnostic and prognosis biomarkers in colon cancer
AU - Martínez-Gutierrez, Antonio
AU - Carbajal-Lopez, Berenice
AU - Bui, Triet M.
AU - Mendoza-Rodriguez, Monica
AU - Campos-Parra, Alma D.
AU - Calderillo-Ruiz, Germán
AU - Cantú-De Leon, David
AU - Madrigal-Santillán, Eduardo Osiris
AU - Sumagin, Ronen
AU - Pérez-Plasencia, Carlos
AU - Pérez-Yépez, Eloy Andrés
N1 - Funding Information:
This work was supported by grants from American Cancer Society Research Scholar Award and Crohn’s & Colitis Foundation Senior Research Award to RS.
Funding Information:
B. C.-L. is a doctoral student from Programa de Doctorado en Investigación en medicina at ESM- IPN and was supported by CONACYT scholarship number 753170 .
Publisher Copyright:
© 2022 The Authors
PY - 2022/7
Y1 - 2022/7
N2 - Colon cancer (CC) is the third most common neoplasm and the fourth cause of cancer-related death worldwide in both sexes. It has been established that inflammation plays a critical role in tumorigenesis and progression of CC. Immune, stromal and tumor cells supply the tumor microenvironment with pro-inflammatory cytokines such as interleukin 1β, TNFα, IL-6 and IL-11, to hyperactivate signaling pathways linked to cancerous processes. Recent findings suggest a putative role of microRNAs (miRNAs) in the progression and management of the inflammatory response in intestinal diseases. Moreover, miRNAs are able to regulate expression of molecular mediators that are linking inflammation and cancer. In this work a miRNA panel differentially expressed between healthy, inflammatory bowel disease (IBD) and CC tissue was established. Identified miRNAs regulate signaling pathways related to inflammation and cancer progression. An inflammation associated-miRNA panel composed of 11-miRNAs was found to be overexpressed in CC but not in inflamed or normal tissues (miR-21-5p, miR-304-5p, miR-577, miR-335-5p, miR-21-3p, miR-27b-5p, miR-335-3p, miR-215-5p, miR-30b-5p, miR-192-5p, miR-3065-5p). The association of top hit miRNAs, miR-3065-5p and miR-30b-5p expression with overall survival of CC patients was demonstrated using Kaplan-Meier tests. Finally, differential miRNA expression was validated using an inflammation-associated CC model induced by Azoxymethane/Dextran Sodium Sulfate (AOM/DSS) to compare miRNA expression in normal and inflamed tissue versus CC tissues. Based on these findings we propose the identified inflammatory miRNA panel as a potent diagnostic tool for CC determination.
AB - Colon cancer (CC) is the third most common neoplasm and the fourth cause of cancer-related death worldwide in both sexes. It has been established that inflammation plays a critical role in tumorigenesis and progression of CC. Immune, stromal and tumor cells supply the tumor microenvironment with pro-inflammatory cytokines such as interleukin 1β, TNFα, IL-6 and IL-11, to hyperactivate signaling pathways linked to cancerous processes. Recent findings suggest a putative role of microRNAs (miRNAs) in the progression and management of the inflammatory response in intestinal diseases. Moreover, miRNAs are able to regulate expression of molecular mediators that are linking inflammation and cancer. In this work a miRNA panel differentially expressed between healthy, inflammatory bowel disease (IBD) and CC tissue was established. Identified miRNAs regulate signaling pathways related to inflammation and cancer progression. An inflammation associated-miRNA panel composed of 11-miRNAs was found to be overexpressed in CC but not in inflamed or normal tissues (miR-21-5p, miR-304-5p, miR-577, miR-335-5p, miR-21-3p, miR-27b-5p, miR-335-3p, miR-215-5p, miR-30b-5p, miR-192-5p, miR-3065-5p). The association of top hit miRNAs, miR-3065-5p and miR-30b-5p expression with overall survival of CC patients was demonstrated using Kaplan-Meier tests. Finally, differential miRNA expression was validated using an inflammation-associated CC model induced by Azoxymethane/Dextran Sodium Sulfate (AOM/DSS) to compare miRNA expression in normal and inflamed tissue versus CC tissues. Based on these findings we propose the identified inflammatory miRNA panel as a potent diagnostic tool for CC determination.
KW - Biomarkers
KW - Colon cancer
KW - Computational assay
KW - Proinflammatory cytokines
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U2 - 10.1016/j.bbrep.2022.101252
DO - 10.1016/j.bbrep.2022.101252
M3 - Article
C2 - 35313644
AN - SCOPUS:85126629646
SN - 2405-5808
VL - 30
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
M1 - 101252
ER -
